Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2279052..2279578 Replicon chromosome
Accession NZ_CP083585
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag LA372_RS11025 Protein ID WP_000323025.1
Coordinates 2279291..2279578 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag LA372_RS11020 Protein ID WP_000534858.1
Coordinates 2279052..2279291 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS10990 (2274414) 2274414..2274903 + 490 Protein_2149 class I SAM-dependent methyltransferase -
LA372_RS10995 (2275381) 2275381..2275802 + 422 Protein_2150 hypothetical protein -
LA372_RS11000 (2276458) 2276458..2277528 + 1071 Protein_2151 ISNCY family transposase -
LA372_RS11005 (2277601) 2277601..2277750 + 150 WP_011443592.1 protein YdfW -
LA372_RS11010 (2278187) 2278187..2278519 - 333 WP_001301033.1 FlxA-like family protein -
LA372_RS11015 (2278722) 2278722..2279027 - 306 WP_001326990.1 protein YdfV -
LA372_RS11020 (2279052) 2279052..2279291 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
LA372_RS11025 (2279291) 2279291..2279578 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
LA372_RS11030 (2279650) 2279650..2279805 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
LA372_RS11035 (2280022) 2280022..2280273 + 252 WP_000980994.1 protein Rem -
LA372_RS11040 (2280340) 2280340..2280618 + 279 WP_012304870.1 hypothetical protein -
LA372_RS11045 (2280620) 2280620..2281669 + 1050 WP_001265198.1 DUF968 domain-containing protein -
LA372_RS11050 (2281683) 2281683..2282435 + 753 WP_001047135.1 antitermination protein -
LA372_RS11055 (2282713) 2282713..2282802 - 90 WP_122985911.1 hypothetical protein -
LA372_RS11060 (2282857) 2282857..2283069 - 213 WP_024172454.1 cold shock-like protein CspF -
LA372_RS11065 (2283368) 2283368..2283583 + 216 WP_000066485.1 cold shock-like protein CspB -
LA372_RS11070 (2283947) 2283947..2284117 - 171 WP_001405948.1 putative zinc-binding protein YnfU -
LA372_RS11075 (2284336) 2284336..2284551 + 216 WP_047624855.1 class II holin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2261899..2314861 52962


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T216784 WP_000323025.1 NZ_CP083585:2279291-2279578 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T216784 NZ_CP113439:c69811-68513 [Legionella pneumophila]
ATGCGTAAATTATACATATTGATGAACAATGAGCTCGTGGGGGAGCTTACCCAGGATAATGCAGGTGGTTTGAGTTTTTC
CTACGTTGCTGACTGGATTTCCAAGCCGCATTCACGCCCAGTTTCTTTATCGTTACCGTTAGTAAATCAAAGGTTTTCAG
GAGAAGTTGTTTATAACTTTTTTGATAATTTACTTCCCGATAATCCACAGATAAGAGCCAGAATTCAAGCTCGTTTTCAG
GCAAAATCATCGCGACCGTTTGATTTATTGGCGTTAATTGGCAGTGATTGTGTTGGTGCAATACAAATTGTTGAACGTAT
TCCTCAGACACAGCGTCTAAGCATTGAAAGTCACGTTGTCAGTGAGGCCGAGATAGCTCGTACTTTACGCTCTTACCAAA
CCTCACCACTGGGAATGGATAAAGAGGATGATGAGTTTAGAATCTCAATAGCCGGTGCTCAGGAAAAAACGGCTTTTCTA
TATTACCAAAATGAATGGCATAAACCATTTGGACCTACACCAACAACTCACATATTCAAATTACCTATTGGATATTTACC
ACATCAAGGAATTGATTTAAGTGAGAGTTGTGAAAATGAGCATCTCTGTTTACTACTAGCCAGCAAATTTGGTTTAGAGG
TTGCAAAATCAAAAATCTTACAATTCGAAGATGAAAAGGTGCTGGTTGTCGAAAGATTTGATAGAAAATGGAGTCGAGAT
AACCGTTATATACTCAGGTTACCGCAAGAAGACCTATGCCAAGCGTTGGGTTATTCACCAAATTTAAAATATCAGTCAGA
TGGTGGCCCTGGTATTAAAGAAATTATGCAGCTACTTAATGGCTCACAGCAGCCTCAAAAAGATAGAGATGAATTTTTCA
GAGCACAAGTTGTTTACTGGTGCCTTGGTGCTATTGATGCGCATGCCAAAAACTTCAGTATAAGTATACAACCACAAGGC
GGTTACCAGCTCACCCCCTTATACGACATCATTTCAGCCTATCCTATCGTGGAACAAAAACAGCTATCCTTTAAGAAATT
GAAAATGGCAATGGCTTTATATGGTAAAAATAGTCATTACAAGATTGACGGTATTCAAAAAAGACACTTTATTGAAACAG
CAAAATACGTTCAATATTCTAAAATTAAAGCAGCTCAGATTTTTGAACAAGTGATTGACCGTATTGATTCTGCTATCGAT
GAGGTCTCAACACAATTACCAAACCACTTCCCAGAAAAGATTGCAGATCCAATCTTCAAAGGGATGAGAGCTTTTAAGGA
GCGAATGATATCCACATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT216784 WP_000534858.1 NZ_CP083585:2279052-2279291 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT216784 NZ_CP113439:c70050-69811 [Legionella pneumophila]
ATGATCATTAGCTCAGCAAAAGATTTAGCATTATATTTAAATGACAAGCGCAAACAACTCAAAATGAGTCAATCTGAAGT
TGCCGATTTAGTCGGCTTAAAACAGGATACCATATCAAAGTTTGAAAATAGCCCTGATAATAGTCGAATTGATACCTTAT
TTAGAATATTGTCCGCCCTAAATTTAAATATGAGCCTTGTTGAAAAAGATCAAAAAACTCATGAAGAGATAGAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References