Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2264682..2265340 Replicon chromosome
Accession NC_008314
Organism Ralstonia eutropha H16

Toxin (Protein)


Gene name vapC Uniprot ID R7XG42
Locus tag H16_RS28775 Protein ID WP_010812752.1
Coordinates 2264682..2265092 (-) Length 137 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R7XF63
Locus tag H16_RS28780 Protein ID WP_010812751.1
Coordinates 2265089..2265340 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H16_RS28755 2260299..2261273 - 975 WP_010812756.1 LysR family transcriptional regulator -
H16_RS28760 2261364..2262593 + 1230 WP_011617587.1 CoA transferase -
H16_RS28765 2262590..2263600 + 1011 WP_010812754.1 NADPH:quinone oxidoreductase family protein -
H16_RS28770 2263654..2264601 + 948 WP_051398610.1 tripartite tricarboxylate transporter substrate binding protein -
H16_RS28775 2264682..2265092 - 411 WP_010812752.1 type II toxin-antitoxin system VapC family toxin Toxin
H16_RS28780 2265089..2265340 - 252 WP_010812751.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H16_RS28785 2265758..2266933 + 1176 WP_011617589.1 branched-chain amino acid ABC transporter substrate-binding protein -
H16_RS28790 2267014..2267943 + 930 WP_013953596.1 branched-chain amino acid ABC transporter permease -
H16_RS28795 2268011..2269141 + 1131 WP_037023464.1 branched-chain amino acid ABC transporter permease -
H16_RS28800 2269138..2269920 + 783 WP_011617591.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

SpoVT-AbrB domain

(8-55)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 14457.92 Da        Isoelectric Point: 8.0357

>T21678 WP_010812752.1 NC_008314:c2265092-2264682 [Cupriavidus necator H16]
MTRYMLDTNIVSLLLRAHPAVVARVTAAPMVALCLSAITGAELMYGLAKRPGAARLARAVDELLRRVEVLPWTPSVMAGY
GETRAALESQGQPVGALNLLIAAHALETGAVLVTNDQAFRRVPGLVVEDWTVPTGN

Download         Length: 411 bp

>T21678 NC_008314:c2265092-2264682 [Cupriavidus necator H16]
ATGACGCGCTACATGCTCGACACCAATATCGTGAGCCTTCTGTTGCGGGCACATCCGGCAGTCGTGGCACGCGTGACGGC
AGCGCCGATGGTGGCGCTGTGCCTGTCAGCCATCACCGGTGCGGAACTGATGTACGGGCTGGCCAAGCGTCCCGGCGCAG
CGCGCCTCGCGCGCGCGGTCGACGAGTTGCTGCGCAGGGTCGAGGTATTGCCCTGGACCCCTTCAGTGATGGCCGGCTAT
GGTGAAACCCGCGCTGCGCTGGAATCCCAAGGACAGCCGGTGGGGGCACTGAACCTGCTGATCGCCGCGCACGCGCTGGA
GACTGGCGCGGTGCTGGTGACCAATGACCAGGCATTCCGGCGCGTCCCTGGCCTGGTGGTGGAGGACTGGACGGTTCCGA
CCGGAAATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9298.38 Da        Isoelectric Point: 4.8011

>AT21678 WP_010812751.1 NC_008314:c2265340-2265089 [Cupriavidus necator H16]
VSTTAKIFTTGRSQAVRLPQEFRFAEREVYIRRDPSTGDVILSRRPESWDGFLAAIKDSAVPEDFMADRAQGEQARDPLA
GLE

Download         Length: 252 bp

>AT21678 NC_008314:c2265340-2265089 [Cupriavidus necator H16]
GTGAGCACCACTGCCAAGATCTTCACCACCGGCCGCAGCCAGGCGGTACGGCTGCCGCAGGAATTCCGCTTCGCCGAGCG
CGAGGTATATATTCGCCGTGACCCGTCCACCGGTGACGTGATCCTGTCGCGCCGGCCGGAGAGTTGGGACGGCTTTTTGG
CTGCCATCAAGGACAGCGCCGTGCCGGAAGACTTTATGGCCGACCGCGCGCAGGGCGAGCAAGCCAGGGATCCGCTGGCG
GGTCTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7T4FZ50

Antitoxin

Source ID Structure
AlphaFold DB A0A7T4FZ54

References