Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 871073..871727 Replicon chromosome
Accession NZ_CP083585
Organism Escherichia coli strain ECNB21-M121

Toxin (Protein)


Gene name cptA Uniprot ID A0A827UDA1
Locus tag LA372_RS04375 Protein ID WP_047623548.1
Coordinates 871320..871727 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LA372_RS04370 Protein ID WP_000354046.1
Coordinates 871073..871339 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LA372_RS04345 (866361) 866361..867104 + 744 WP_053273601.1 SDR family oxidoreductase -
LA372_RS04350 (867161) 867161..868594 - 1434 WP_224204990.1 6-phospho-beta-glucosidase BglA -
LA372_RS04355 (868639) 868639..868950 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
LA372_RS04360 (869114) 869114..869773 + 660 WP_000250274.1 hemolysin III family protein -
LA372_RS04365 (869850) 869850..870830 - 981 WP_000886074.1 tRNA-modifying protein YgfZ -
LA372_RS04370 (871073) 871073..871339 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LA372_RS04375 (871320) 871320..871727 + 408 WP_047623548.1 protein YgfX Toxin
LA372_RS04380 (871767) 871767..872288 - 522 WP_001055874.1 flavodoxin FldB -
LA372_RS04385 (872400) 872400..873296 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LA372_RS04390 (873321) 873321..874031 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LA372_RS04395 (874037) 874037..875770 + 1734 WP_047623549.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T216774 WP_047623548.1 NZ_CP083585:871320-871727 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGIMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216774 NZ_CP113432:1830695-1831456 [Pseudomonas sp. ZM23]
ATGGAAGGTGCCGAGATCTGGGCGAGGCACGAGCAGGACGCCGTCGTAGGGCCGATTCGCGGCCGCCTCGTGCGGCTGGT
GGAAAGCCAGGGGCAGGTGGCCACCCTGCAACTGGTGGATACCCTTGAGGAGCAGGCGCTGCTGGAGGAGTTGCTGGAAA
CCAGCAAACCCCGCGTGCCGCCGTCCGCCGAGAGCCTGCACTATCTGCTGAAAACGCCGTTTCGCTACCCCCCGCTGCGC
TGGGGCTCGCGCTTTGGCCGGCGCCATGAGCCGAGTCTGTTCTATGCGGCACTCAAGCTGGAGACGGCGATGGCCGAGTC
GGCCTATTACCGCTTCGTTCTCTGGGAGGGCATGAGCGCGCCGCCGCCCAGCGGCCGGATTCTCTCCGAACATGCGTCGT
TCGAGGCGCGCTTCCAGGTACAGCGTGGCGTGCGCCTGCAATTGCCGCCCTTCAGCGAGTACGAGGCCGAGCTTTGCGAC
CCGGCGGACTACCGGGTTACCCAGGGCCTGGGCAGTTCAATGCGGGAAGCCGGGGTCGAGGCGTTCGAGTACCGCTCTGC
GCGCTGCCCCCAGCGCGGGGTGAACGTGGCGTTGTACGCACCCTCGGCGTTCATGGAGAAGCGCCCGCGCAACCTGGCGC
CCTGGCTGTGCGAGACCACCGCAGAGTATGTGGCATTCAAGCACGGACAGGTGCCGGACACGCCGCGCGTGTTCACGCGC
GAGCGATATCTGGTGGACGGGCGGCTGCCGTACCCGGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216774 WP_000354046.1 NZ_CP083585:871073-871339 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216774 NZ_CP113432:1830326-1830694 [Pseudomonas sp. ZM23]
ATGAGCGCACTCTTGCGTGAAAGCCCTGCCGCGGATGCCGTTCTGGCCAAGGCGGTTCTCACTGCCCGCGAACACCTGGC
GATGACCCAGCAGGAGCTGGCGGCCATTGTCGGTGTGGATCGCAGCGCTGTCAGCCGCTGGAAGCAGGGCGGCTTGCGGG
TGGACAGCAAGACCGGTGAACTGGCGTTGCTGCTGGTACGCATCTATCGGGCCCTGTTCGCCCTGTTCGGCGGCAATCTT
GAAGACATGCGCCATTTCCTGCGTACCGAGAACCGACATCTGGGCGGTGTTCCACTGCAACTTATGGAGCGGGTGCAGGG
GCTGGTAACGGTGATCGAGTACCTCGATGCCATTCGGGGCAAGGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A827UDA1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References