Detailed information of TA system TA216773

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 766660..767494
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: LA372_RS03820
• Protein ID: WP_089613745.1
• Coordinates: 766660..767037 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LA372_RS03825
• Protein ID: WP_089613744.1
• Coordinates: 767126..767494 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS03790 (763134)	763134..763730	+	597	Protein_744	DUF2220 family protein	-
LA372_RS03795 (764357)	764357..764683	-	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LA372_RS03800 (764680)	764680..764943	-	264	WP_001143297.1	type II toxin-antitoxin system ParD family antitoxin	-
LA372_RS03805 (765015)	765015..765881	-	867	WP_032251878.1	DUF4942 domain-containing protein	-
LA372_RS03810 (765966)	765966..766163	-	198	WP_089613747.1	DUF957 domain-containing protein	-
LA372_RS03815 (766175)	766175..766663	-	489	WP_089613746.1	DUF5983 family protein	-
LA372_RS03820 (766660)	766660..767037	-	378	WP_089613745.1	TA system toxin CbtA family protein	Toxin
LA372_RS03825 (767126)	767126..767494	-	369	WP_089613744.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LA372_RS03830 (767574)	767574..767795	-	222	WP_000692323.1	DUF987 domain-containing protein	-
LA372_RS03835 (767858)	767858..768334	-	477	WP_074503008.1	RadC family protein	-
LA372_RS03840 (768350)	768350..768835	-	486	WP_000206660.1	antirestriction protein	-
LA372_RS03845 (768927)	768927..769745	-	819	WP_001234693.1	DUF932 domain-containing protein	-
LA372_RS03850 (769844)	769844..770077	-	234	WP_001278281.1	DUF905 family protein	-
LA372_RS03855 (770083)	770083..770760	-	678	WP_001097362.1	hypothetical protein	-
LA372_RS03860 (770879)	770879..771763	-	885	WP_224204954.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	751620..781111	29491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14287.35 Da
• Isoelectric Point: 9.7064

>T216773 WP_089613745.1 NZ_CP083585:c767037-766660 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTGQPRF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKR

Nucleotide

• Length: 378 bp

>T216773 NZ_CP113432:1827121-1827462 [Pseudomonas sp. ZM23]
ATGACAATCCTCGATGTCCGTTTCACCGAGACAGCAGTCTTCAGTATTCAGGATCAGGAAGAGCACCTGGCCGAGTACCA
TCCTGCCGAGGTGGCTGCAGCCAAGATCGATACGTTGATCGATGACATTCTGACCAGGCTGCAGAATGCCCCTGTCGGCT
ACCCGGTCAGCCGCCAGGCGAGCGACCTGGGTGTCACTCGTTACCGTGAGCTTAATCACGACGGATACCGTGTGTTCTAC
GAAGTGTATGAGCACGACAATGCGATTGCAGTCGAGCTGGTCCTGCGGCAGAAACAGGACGTGGAAGCGGCTCTGATCCG
CTATTGCCTGGTCGGCATCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13648.42 Da
• Isoelectric Point: 7.7474

>AT216773 WP_089613744.1 NZ_CP083585:c767494-767126 [Escherichia coli]
VSDTFSGTTHPDDNRGRPWWGLPCTVTPCFGARLVQESNQLHYLANRAGIRGRFSDADAYHLDQTFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLGSCGYVYLAVYPTSETKK

Nucleotide

• Length: 369 bp

>AT216773 NZ_CP113432:1826865-1827113 [Pseudomonas sp. ZM23]
ATGCGGGTTGAAACGATCAGCTACCTGAAGCGGCATGCTGCGGATCTGGAGCTGGACGAGCCGATGGTCGTCACGCAGAA
CGGCGTACCGACAATGGTGGTGGAGTCCTACGCAGAGCGTAAGCGCCGTGATCAGGCGATAGCCCTGGTCAAGCTGCTGG
CGATGGGATCGCGCGAGTACACTGAAGGTAAGCATTGCTCTGCTGATGAGCTGAAGGCCCGTCTGGCCAAAAGACGCACT
CAGCAGTAA