Detailed information of TA system TA216770

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 347050..347804
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LA372_RS01640
• Protein ID: WP_001301452.1
• Coordinates: 347050..347535 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: LA372_RS01645
• Protein ID: WP_000801914.1
• Coordinates: 347526..347804 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS01620 (342217)	342217..342975	+	759	WP_000815099.1	DeoR/GlpR family transcriptional regulator	-
LA372_RS01625 (342957)	342957..344555	-	1599	WP_047624737.1	DNA-binding transcriptional regulator RtcR	-
LA372_RS01630 (344742)	344742..345968	+	1227	WP_001105465.1	RNA-splicing ligase RtcB	-
LA372_RS01635 (345972)	345972..347000	+	1029	WP_024257792.1	RNA 3'-terminal phosphate cyclase	-
LA372_RS01640 (347050)	347050..347535	-	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
LA372_RS01645 (347526)	347526..347804	-	279	WP_000801914.1	DUF1778 domain-containing protein	Antitoxin
LA372_RS01650 (347871)	347871..350576	-	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T216770 WP_001301452.1 NZ_CP083585:c347535-347050 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T216770 NZ_CP113428:245498-245848 [Bacillus cereus]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10406.91 Da
• Isoelectric Point: 4.6336

>AT216770 WP_000801914.1 NZ_CP083585:c347804-347526 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFIVDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT216770 NZ_CP113428:245206-245493 [Bacillus cereus]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA