Detailed information of TA system TA216769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 299955..300755
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: LA372_RS01435
• Protein ID: WP_047624754.1
• Coordinates: 300228..300755 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: U9Y285
• Locus tag: LA372_RS01430
• Protein ID: WP_001277109.1
• Coordinates: 299955..300221 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS01410 (295613)	295613..296281	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
LA372_RS01415 (296274)	296274..297332	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
LA372_RS01420 (297577)	297577..298431	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
LA372_RS01425 (298702)	298702..299805	+	1104	WP_077782892.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
LA372_RS01430 (299955)	299955..300221	+	267	WP_001277109.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
LA372_RS01435 (300228)	300228..300755	+	528	WP_047624754.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
LA372_RS01440 (300752)	300752..301135	-	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
LA372_RS01445 (301559)	301559..302668	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
LA372_RS01450 (302716)	302716..303642	+	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LA372_RS01455 (303639)	303639..304916	+	1278	WP_085459965.1	branched chain amino acid ABC transporter permease LivM	-
LA372_RS01460 (304913)	304913..305680	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19672.69 Da
• Isoelectric Point: 7.7668

>T216769 WP_047624754.1 NZ_CP083585:300228-300755 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENKNAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T216769 NZ_CP113418:c1242121-1241375 [Bacillus amyloliquefaciens]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTGAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCTGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCAGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9938.37 Da
• Isoelectric Point: 10.0863

>AT216769 WP_001277109.1 NZ_CP083585:299955-300221 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Nucleotide

• Length: 267 bp

>AT216769 NZ_CP113418:c1241374-1241204 [Bacillus amyloliquefaciens]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LUN0