Detailed information of TA system TA216768

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 262877..263508
• Replicon: chromosome
• Accession: NZ_CP083585
• Organism: Escherichia coli strain ECNB21-M121

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LA372_RS01215
• Protein ID: WP_047623515.1
• Coordinates: 262877..263152 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: LA372_RS01220
• Protein ID: WP_000593555.1
• Coordinates: 263149..263508 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LA372_RS01200 (257881)	257881..258948	+	1068	WP_000361491.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LA372_RS01205 (258945)	258945..261680	+	2736	WP_000149130.1	ribosome-associated ATPase/putative transporter RbbA	-
LA372_RS01210 (261680)	261680..262804	+	1125	WP_001314210.1	ABC transporter permease	-
LA372_RS01215 (262877)	262877..263152	+	276	WP_047623515.1	type II toxin-antitoxin system HicA family toxin	Toxin
LA372_RS01220 (263149)	263149..263508	+	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LA372_RS01225 (263628)	263628..264029	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
LA372_RS01230 (264035)	264035..264841	-	807	WP_047623514.1	nickel import ATP-binding protein NikE	-
LA372_RS01235 (264838)	264838..265602	-	765	WP_047623513.1	nickel import ATP-binding protein NikD	-
LA372_RS01240 (265602)	265602..266435	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
LA372_RS01245 (266432)	266432..267376	-	945	WP_047623512.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	254042..263508	9466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10257.98 Da
• Isoelectric Point: 10.5488

>T216768 WP_047623515.1 NZ_CP083585:262877-263152 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKELGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T216768 NZ_CP113418:490251-490601 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT216768 WP_000593555.1 NZ_CP083585:263149-263508 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT216768 NZ_CP113418:489964-490245 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6