Detailed information of TA system TA216767

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 561818..562704
• Replicon: plasmid pA
• Accession: NZ_CP083583
• Organism: Kovacikia minuta CCNUW1 strain CCNU0001

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: -
• Locus tag: K9N68_RS36415
• Protein ID: WP_224346625.1
• Coordinates: 562252..562704 (+)
• Length: 151 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: -
• Locus tag: K9N68_RS36410
• Protein ID: WP_224346624.1
• Coordinates: 561818..562255 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9N68_RS36390 (K9N68_36390)	557279..557452	-	174	WP_224346620.1	hypothetical protein	-
K9N68_RS36395 (K9N68_36395)	557542..558252	-	711	WP_224346621.1	WYL domain-containing protein	-
K9N68_RS36400 (K9N68_36400)	558838..559626	+	789	WP_224346622.1	hypothetical protein	-
K9N68_RS36405 (K9N68_36405)	559623..561629	+	2007	WP_224346623.1	patatin-like phospholipase family protein	-
K9N68_RS36410 (K9N68_36410)	561818..562255	+	438	WP_224346624.1	nucleotidyltransferase domain-containing protein	Antitoxin
K9N68_RS36415 (K9N68_36415)	562252..562704	+	453	WP_224346625.1	DUF86 domain-containing protein	Toxin
K9N68_RS36420 (K9N68_36420)	562710..563657	+	948	WP_224346626.1	CRISPR-associated endoribonuclease Cas6	-
K9N68_RS36425 (K9N68_36425)	563717..563878	+	162	WP_224346627.1	hypothetical protein	-
K9N68_RS36430 (K9N68_36430)	563871..564464	+	594	WP_224346628.1	CRISPR-associated protein Cas4	-
K9N68_RS36435 (K9N68_36435)	564600..565622	+	1023	WP_224346629.1	type I-D CRISPR-associated endonuclease Cas1d	-
K9N68_RS36440 (K9N68_36440)	565805..566077	+	273	WP_224346630.1	CRISPR-associated endonuclease Cas2	-
K9N68_RS36445 (K9N68_36445)	566099..566287	+	189	WP_224346631.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..1207795	1207795

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (19-140)

Antitoxin

• Polymerase beta, nucleotidyltransferase (14-106)

Sequences

Toxin

Protein

• Length: 151 a.a.
• Molecular weight: 16963.31 Da
• Isoelectric Point: 4.2700

>T216767 WP_224346625.1 NZ_CP083583:562252-562704 [Kovacikia minuta CCNUW1]
MTRLDPEAIAQKLSRMVDRIDRLKLFEGLPLEEYLQDDLKQAAIERLLEAIIDAALSINKTLLRRGAGIIPADSESFKNF
ESFILMAENGFITVELANQLAPSGSFRNVLAHEYDDIDPTQVYNALQKALTQYPQYVKAIQSYLDALEED

Nucleotide

• Length: 453 bp

>T216767 NZ_CP113364:4558492-4558803 [Salmonella enterica subsp. enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16268.54 Da
• Isoelectric Point: 5.6231

>AT216767 WP_224346624.1 NZ_CP083583:561818-562255 [Kovacikia minuta CCNUW1]
VTTLDSLKAAFSTLASTHCDLKLLVLFGSRTRGEADPSSDWDFAFLLEPDRPVELKPFWFPGSNLLGTLCNLLHVSEDAI
DLVDLSTCSEITAHFVARDGQVIYEKIAGEFAQFQGRALKTKAELKQYRHAQREKVLQTLERWGV

Nucleotide

• Length: 438 bp

>AT216767 NZ_CP113364:4558800-4559246 [Salmonella enterica subsp. enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCAGATGCCTTCATCGAGTAG