Detailed information of TA system TA216766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 7224614..7225233
• Replicon: chromosome
• Accession: NZ_CP083582
• Organism: Kovacikia minuta CCNUW1 strain CCNU0001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K9N68_RS33560
• Protein ID: WP_224342452.1
• Coordinates: 7224614..7225009 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K9N68_RS33565
• Protein ID: WP_224342453.1
• Coordinates: 7225009..7225233 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9N68_RS33530 (K9N68_33530)	7220098..7221291	-	1194	WP_224342446.1	Coenzyme F420 hydrogenase/dehydrogenase, beta subunit C-terminal domain	-
K9N68_RS33535 (K9N68_33535)	7221486..7221644	-	159	WP_224342447.1	hypothetical protein	-
K9N68_RS33540 (K9N68_33540)	7221725..7222141	-	417	WP_224342448.1	PIN domain-containing protein	-
K9N68_RS33545 (K9N68_33545)	7222141..7222362	-	222	WP_224342449.1	hypothetical protein	-
K9N68_RS33550 (K9N68_33550)	7222616..7223218	-	603	WP_224342450.1	Uma2 family endonuclease	-
K9N68_RS33555 (K9N68_33555)	7223226..7224617	-	1392	WP_224342451.1	DNA methyltransferase	-
K9N68_RS33560 (K9N68_33560)	7224614..7225009	-	396	WP_224342452.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9N68_RS33565 (K9N68_33565)	7225009..7225233	-	225	WP_224342453.1	DUF2281 domain-containing protein	Antitoxin
K9N68_RS33570 (K9N68_33570)	7225233..7226972	-	1740	WP_224342454.1	N-6 DNA methylase	-
K9N68_RS33575 (K9N68_33575)	7227506..7227928	-	423	WP_224342455.1	hypothetical protein	-
K9N68_RS33580 (K9N68_33580)	7228136..7228387	+	252	WP_224342456.1	hypothetical protein	-
K9N68_RS33585 (K9N68_33585)	7228411..7229592	-	1182	WP_224342457.1	MFS transporter	-
K9N68_RS33590 (K9N68_33590)	7229589..7230188	-	600	WP_224342458.1	DJ-1/PfpI family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Domain of unknown function DUF2281 (6-73)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 15168.57 Da
• Isoelectric Point: 4.5849

>T216766 WP_224342452.1 NZ_CP083582:c7225009-7224614 [Kovacikia minuta CCNUW1]
MSAVLLDTHAFIWLVEDSPKLPQPIKERIAVTNDIFVSIASFWEIGIKLKKAQIFLSCDFNEIESRFLETDLQLLPISIQ
DTIQQYNLPFYLAEHKDPFDRIIISQAISRSLPLVSKDKEFDAYPIEKVWA

Nucleotide

• Length: 396 bp

>T216766 NZ_CP113364:c4557259-4556948 [Salmonella enterica subsp. enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGTTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGATCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCCTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8697.16 Da
• Isoelectric Point: 4.8428

>AT216766 WP_224342453.1 NZ_CP083582:c7225233-7225009 [Kovacikia minuta CCNUW1]
MIKPTILEKLEKLPEALQTEVLHYIEFLLEKHTTPPIQKETPKKRRLAGSMKGTFVLPLPDNFDEPLEEFEDYM

Nucleotide

• Length: 225 bp

>AT216766 NZ_CP113364:c4556947-4556657 [Salmonella enterica subsp. enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA