Detailed information of TA system TA216764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RHH
• Location: 586472..586992
• Replicon: chromosome
• Accession: NZ_CP083582
• Organism: Kovacikia minuta CCNUW1 strain CCNU0001

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K9N68_RS02740
• Protein ID: WP_224342997.1
• Coordinates: 586472..586759 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K9N68_RS02745
• Protein ID: WP_224342998.1
• Coordinates: 586756..586992 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9N68_RS02720 (K9N68_02720)	582092..582505	+	414	WP_224342993.1	response regulator	-
K9N68_RS02725 (K9N68_02725)	582920..583486	+	567	WP_224342994.1	Hsp20/alpha crystallin family protein	-
K9N68_RS02730 (K9N68_02730)	583768..585681	+	1914	WP_224342995.1	molecular chaperone DnaK	-
K9N68_RS02735 (K9N68_02735)	585824..586399	-	576	WP_224342996.1	TIGR00730 family Rossman fold protein	-
K9N68_RS02740 (K9N68_02740)	586472..586759	-	288	WP_224342997.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9N68_RS02745 (K9N68_02745)	586756..586992	-	237	WP_224342998.1	ribbon-helix-helix protein, CopG family	Antitoxin
K9N68_RS02750 (K9N68_02750)	587366..588694	-	1329	WP_224342999.1	DUF58 domain-containing protein	-
K9N68_RS02755 (K9N68_02755)	588788..589738	-	951	WP_224343000.1	MoxR family ATPase	-
K9N68_RS02760 (K9N68_02760)	589844..591019	-	1176	WP_224343002.1	DUF4350 domain-containing protein	-
K9N68_RS02765 (K9N68_02765)	591101..591709	-	609	WP_224343003.1	DUF4129 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11036.72 Da
• Isoelectric Point: 10.0332

>T216764 WP_224342997.1 NZ_CP083582:c586759-586472 [Kovacikia minuta CCNUW1]
MRIIWTQQAISDLNHAYDYIAETAPESGTAIIERIEKSLEALSNFPEMGRKGRVNNTRELIVPKTPFILPYRIRKNRIEI
LGVIHATRRWPSSFE

Nucleotide

• Length: 288 bp

>T216764 NZ_CP113364:4102301-4102717 [Salmonella enterica subsp. enterica]
ATGAGTAAAACCTACATGCTGGATACCTGCATCTGTTCTTTCATTATGCGCGAACAGCCGGAAGCTGTGCTGAAACGCCT
TGAGCAGGCAGTGCTGCGCCGCCATCGTATTGTGGTGTCGGCGATTACCTACGCCGAAATGCGTTTTGGCTGTACGGGCA
AAAAGGCCTCGCCGCGCCATGCCCAACTGGTTGATGCATTCTGTAGTCGCCTGGATGCCGTGCTGGCCTGGGACCGGGCG
GCAGTGGATGCCACCACAGAGATCCGTGCCGTACTGGCCGCCGTCGGTACGCCGATTGGATCAAACGATGCGGCTATCGC
CGGCCATGCCATTGCGTCCGGGGCGATACTGGTCACTAATAACGTGAGGGAGTTTGAGCGGGTACCGGGTTTGCAGTACG
AAGATTGGGTAAAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9066.08 Da
• Isoelectric Point: 4.3009

>AT216764 WP_224342998.1 NZ_CP083582:c586992-586756 [Kovacikia minuta CCNUW1]
MSKETITFRLDQEKRAALDEIAEGMDRDRSYILNEAIDLYLEVHHWQVEHILAGMQQADAEEFATDAEVAAAFARWRQ

Nucleotide

• Length: 237 bp

>AT216764 NZ_CP113364:4102074-4102304 [Salmonella enterica subsp. enterica]
ATGCGAACCGTATCTGTATTTAAAAATGGCAATAACCGCGCGATCCGCCTTCCACGCGATCTGGATTTTGACGGCGTCAA
CGAACTTGAAATCTTCCGCGAAGGCGACACGATTATACTGCGCCCCGCCCGCCCTTCCTGGGGATCATTCCGTCATGAGG
AAAAAGCCAGCCCAGACTTTCTGACGGAACGCGAAGACATCATCAGCGATGAGGGACGCTTTGAACTATGA