Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 624311..624947 Replicon chromosome
Accession NZ_CP083580
Organism Bacillus sp. PM8313

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag K9N56_RS03100 Protein ID WP_003179128.1
Coordinates 624597..624947 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag K9N56_RS03095 Protein ID WP_006638778.1
Coordinates 624311..624592 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9N56_RS03075 (K9N56_03075) 619441..620922 + 1482 WP_040348458.1 PH domain-containing protein -
K9N56_RS03080 (K9N56_03080) 620919..621518 - 600 WP_006638775.1 rhomboid family intramembrane serine protease -
K9N56_RS03085 (K9N56_03085) 621800..622816 + 1017 WP_087962525.1 outer membrane lipoprotein carrier protein LolA -
K9N56_RS03090 (K9N56_03090) 623031..624206 + 1176 WP_006638777.1 alanine racemase -
K9N56_RS03095 (K9N56_03095) 624311..624592 + 282 WP_006638778.1 hypothetical protein Antitoxin
K9N56_RS03100 (K9N56_03100) 624597..624947 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K9N56_RS03105 (K9N56_03105) 625067..625894 + 828 WP_006638779.1 RsbT co-antagonist protein RsbRA -
K9N56_RS03110 (K9N56_03110) 625898..626263 + 366 WP_006638780.1 STAS domain-containing protein -
K9N56_RS03115 (K9N56_03115) 626266..626667 + 402 WP_006638781.1 anti-sigma regulatory factor -
K9N56_RS03120 (K9N56_03120) 626678..627685 + 1008 WP_006638782.1 PP2C family protein-serine/threonine phosphatase -
K9N56_RS03125 (K9N56_03125) 627744..628070 + 327 WP_006638783.1 anti-sigma factor antagonist -
K9N56_RS03130 (K9N56_03130) 628070..628552 + 483 WP_006638784.1 anti-sigma B factor RsbW -
K9N56_RS03135 (K9N56_03135) 628518..629309 + 792 WP_006638785.1 RNA polymerase sigma factor SigB -
K9N56_RS03140 (K9N56_03140) 629306..629905 + 600 WP_006638786.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T216762 WP_003179128.1 NZ_CP083580:624597-624947 [Bacillus sp. PM8313]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T216762 NZ_CP113364:3589636-3589842 [Salmonella enterica subsp. enterica]
ATTCCCGAACTGCATCTTAAGGGCGACTGGCTGGCCGAGGCGGGATTTGAGACCGGGACGAGCGTAACCGTGAAGATCTC
GGAGGGGTGTTTGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCTGGTGAAAAAGTCGA
TGAAGCATATTAAGGCGGGGGTAAATAATGTGGTGAACAGGGATTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT216762 WP_006638778.1 NZ_CP083580:624311-624592 [Bacillus sp. PM8313]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT216762 NZ_CP113364:c3589832-3589647 [Salmonella enterica subsp. enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCAGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCCGAGATCTTCACGGTTACGCTCGTCCCGGTCTCAAATCCCGCCT
CGGCCAGCCAGTCGCCCTTAAGATGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References