Detailed information of TA system TA216757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 138547..140170
• Replicon: plasmid unnamed2
• Accession: NZ_CP083532
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: K9U05_RS24995
• Protein ID: WP_228613871.1
• Coordinates: 138845..140170 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: K9U05_RS24990
• Protein ID: WP_024136327.1
• Coordinates: 138547..138840 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS24965 (K9U05_24975)	134419..135660	+	1242	WP_228613865.1	VWA domain-containing protein	-
K9U05_RS25770	135751..135882	+	132	WP_000374059.1	hypothetical protein	-
K9U05_RS24970 (K9U05_24980)	136223..136504	-	282	WP_000077926.1	hypothetical protein	-
K9U05_RS24975 (K9U05_24985)	136554..136745	-	192	WP_000398480.1	hypothetical protein	-
K9U05_RS24980 (K9U05_24990)	136837..137178	-	342	WP_228613867.1	hypothetical protein	-
K9U05_RS24985 (K9U05_24995)	137551..137943	+	393	WP_228613869.1	hypothetical protein	-
K9U05_RS24990 (K9U05_25000)	138547..138840	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
K9U05_RS24995 (K9U05_25005)	138845..140170	+	1326	WP_228613871.1	type II toxin-antitoxin system HipA family toxin	Toxin
K9U05_RS25000 (K9U05_25010)	140231..140437	+	207	WP_000134171.1	hypothetical protein	-
K9U05_RS25005 (K9U05_25015)	140539..140949	+	411	WP_000985911.1	hypothetical protein	-
K9U05_RS25010 (K9U05_25020)	140962..141777	+	816	WP_228613873.1	HNH endonuclease	-
K9U05_RS25015 (K9U05_25025)	142031..142456	+	426	WP_228613875.1	hypothetical protein	-
K9U05_RS25020 (K9U05_25030)	143005..143313	+	309	WP_001224687.1	hypothetical protein	-
K9U05_RS25025 (K9U05_25035)	143329..144186	+	858	WP_228613877.1	HNH endonuclease signature motif containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / tet(A) / blaTEM-1B / sul3 / ant(3'')-Ia	-	1..268672	268672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (155-404)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50331.29 Da
• Isoelectric Point: 5.8810

>T216757 WP_228613871.1 NZ_CP083532:138845-140170 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMNATDDYAKNFS
ISIGPQGRYHLTPNYDVLSALPVIGHGNNQISWQKCKLAMVVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T216757 NZ_CP113364:c2027178-2026867 [Salmonella enterica subsp. enterica]
ATGCATGTCATATCAAAAGAGCCTTTTGATGAAGCGGCAAGACGATATCCCAATGACTCGTTAGCCATAAAAGCCCTGTA
TCGCTTAGTCCGCGAAAGAGATTTCTCTTCTCCGGCAGAGCTACGAAAAGTCATACCGAGTCTCGATAACTTTAAGTACC
GTAACAAGTGGTGGGTACTGGATGTCGGAGGCAACAACCTGAGGGTGATCGCTTACATCAACTTTATAAACAAACGCTTT
TATGTGAAACACATCGCCACTCACGCTGAATATGACAAGCTGACCCGCTACTACAGGGAGAATAAAGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT216757 WP_024136327.1 NZ_CP083532:138547-138840 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT216757 NZ_CP113364:c2026870-2026451 [Salmonella enterica subsp. enterica]
ATGATTGCCAACACCGCAAAAGCTATCGAAGCCACAAAGGCGCTGGTCGCCGCCGTTCCTTTCCTGGGTGGCAGCGCGTC
AGAGAAAGATTATCGTGATGCGCTAGCGCTGGTTGATTATCTTATCGAAAATGACGATGAAAACCCGCTGATTGACTTCC
TGGCAAGCAAAATTGCCGAGTACGAAGATAACAGCAAGCAGTTCGCCGAGTTCAACAAATCTGTTGCTGAAATGCCGGTT
GGTGTTGCCCTGCTGCGCACGCTAATTGACCAGTACAAACTCTCATACTCAGATCTTAAAGAAGAAATCGGTTCCAAATC
ACTGGTAAGTCAGATCCTGTCCGGACAGCGCTCGTTAACGATTACGCACATCAAAGCGCTTTCAGCACGATTTGGCGTGA
AACCTGAGTGGTTCTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1