Detailed information of TA system TA216756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 23378..23900
• Replicon: plasmid unnamed2
• Accession: NZ_CP083532
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K9U05_RS24450
• Protein ID: WP_228613953.1
• Coordinates: 23619..23900 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K9U05_RS24445
• Protein ID: WP_228613951.1
• Coordinates: 23378..23629 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS24425 (K9U05_24435)	20153..20320	-	168	WP_228614010.1	hypothetical protein	-
K9U05_RS24430 (K9U05_24440)	20641..20823	+	183	WP_042634467.1	hypothetical protein	-
K9U05_RS24435 (K9U05_24445)	20857..21561	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
K9U05_RS24445 (K9U05_24455)	23378..23629	+	252	WP_228613951.1	plasmid stabilization protein	Antitoxin
K9U05_RS24450 (K9U05_24460)	23619..23900	+	282	WP_228613953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9U05_RS24455 (K9U05_24465)	24139..24231	-	93	Protein_33	IS6 family transposase	-
K9U05_RS24460 (K9U05_24470)	24397..25596	-	1200	WP_228613955.1	IS91 family transposase	-
K9U05_RS24465 (K9U05_24475)	25606..25794	-	189	WP_000957857.1	hypothetical protein	-
K9U05_RS24470 (K9U05_24480)	25914..27344	+	1431	Protein_36	IS4-like element IS50R family transposase	-
K9U05_RS24475 (K9U05_24485)	27373..28167	+	795	WP_228613957.1	APH(3')-II family aminoglycoside O-phosphotransferase	-
K9U05_RS24480 (K9U05_24490)	28188..28424	+	237	Protein_38	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-IIa / tet(A) / blaTEM-1B / sul3 / ant(3'')-Ia	-	1..268672	268672
	-	inside	IScluster/Tn	aph(3')-IIa	-	21642..30074	8432

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11129.99 Da
• Isoelectric Point: 10.4889

>T216756 WP_228613953.1 NZ_CP083532:23619-23900 [Escherichia coli]
MTYELAFDRRALKEWQKLYHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Nucleotide

• Length: 282 bp

>T216756 NZ_CP113364:2019869-2019972 [Salmonella enterica subsp. enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9224.45 Da
• Isoelectric Point: 4.1683

>AT216756 WP_228613951.1 NZ_CP083532:23378-23629 [Escherichia coli]
MSYQILTTTATSITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT216756 NZ_CP113364:c2019974-2019831 [Salmonella enterica subsp. enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAAGTTTTCCAGTTTG