Detailed information of TA system TA216755

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 149901..150544
• Replicon: plasmid unnamed1
• Accession: NZ_CP083531
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K9U05_RS24270
• Protein ID: WP_021512278.1
• Coordinates: 150128..150544 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: V0SR71
• Locus tag: K9U05_RS24265
• Protein ID: WP_001261278.1
• Coordinates: 149901..150131 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS24235 (145411)	145411..145716	-	306	WP_228613821.1	type II toxin-antitoxin system toxin CcdB	-
K9U05_RS24240 (145718)	145718..145936	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
K9U05_RS24245 (146504)	146504..147016	+	513	WP_000151784.1	hypothetical protein	-
K9U05_RS24250 (147050)	147050..148183	-	1134	WP_228613823.1	DUF3800 domain-containing protein	-
K9U05_RS24255 (148350)	148350..149123	-	774	WP_228613825.1	hypothetical protein	-
K9U05_RS24260 (149136)	149136..149636	-	501	WP_000528932.1	HEPN family nuclease	-
K9U05_RS24265 (149901)	149901..150131	+	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K9U05_RS24270 (150128)	150128..150544	+	417	WP_021512278.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9U05_RS24275 (150589)	150589..154383	-	3795	WP_001144731.1	hypothetical protein	-
K9U05_RS24280 (154764)	154764..154994	+	231	WP_001261286.1	type II toxin-antitoxin system VapB family antitoxin	-
K9U05_RS24285 (154991)	154991..155407	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / dfrA14	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..155994	155994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15008.34 Da
• Isoelectric Point: 6.7113

>T216755 WP_021512278.1 NZ_CP083531:150128-150544 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSTITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Nucleotide

• Length: 417 bp

>T216755 NZ_CP113364:c968160-967633 [Salmonella enterica subsp. enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGAAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGCTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8853.07 Da
• Isoelectric Point: 4.6437

>AT216755 WP_001261278.1 NZ_CP083531:149901-150131 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Nucleotide

• Length: 231 bp

>AT216755 NZ_CP113364:c968447-968157 [Salmonella enterica subsp. enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCGGCCGGACATGAAGAAATGA

Structures

Antitoxin

• AlphaFold DB: V0SR71