Detailed information of TA system TA216750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 99939..100564
• Replicon: plasmid unnamed1
• Accession: NZ_CP083531
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K9U05_RS23940
• Protein ID: WP_228613800.1
• Coordinates: 100166..100564 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: K9U05_RS23935
• Protein ID: WP_000450520.1
• Coordinates: 99939..100166 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS23935 (99939)	99939..100166	+	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K9U05_RS23940 (100166)	100166..100564	+	399	WP_228613800.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K9U05_RS23945 (100573)	100573..102726	-	2154	WP_228613801.1	type IV conjugative transfer system coupling protein TraD	-
K9U05_RS23950 (102979)	102979..103710	-	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
K9U05_RS23955 (103742)	103742..104239	-	498	WP_000605857.1	entry exclusion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / dfrA14	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN / vat	1..155994	155994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14931.18 Da
• Isoelectric Point: 7.8605

>T216750 WP_228613800.1 NZ_CP083531:100166-100564 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
SQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T216750 NZ_CP113343:1157887-1158144 [Arthrobacter sp. MMS18-M83]
GTGAGATACAACGTCGTGGTCCTGCCGGCGGCCCTTCGGCAGTTGCGCAAGCTTCCGCCCGAGGCGCGTCGACGGATCCA
AGCAGCCATCGAGTTGCTTGCCGAGACACCGCGCCCCCCTGGCGCCAAAAAGCTAACAGGACGGGTCGAATGGCGGGTTC
GTACCGGGGATTATCGGGTGCTCTACCGGATCGACGACGGCCGGCTCGTCATCGTCGTCGTTCAGGCCGGTCATCGCCGC
GAAGTCTACCGGGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT216750 WP_000450520.1 NZ_CP083531:99939-100166 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT216750 NZ_CP113343:1157624-1157890 [Arthrobacter sp. MMS18-M83]
ATGACCGACATAGCCATCACCGAAGCCCGGAACCATCTGGCAACCATTGTGGACACCGCACATCGCGAGCACGAACCGGT
ATTCCTCACGCGCCGCGGAATTCGGGTCGCCGCAGTGATTGATTCCGCCGAACTGGAGCGCCTTCGTGAAGCCGCGGAAG
AACTTGAAGACATCCGGGCCGTCGATTTGGCATGGGAAGAGATGGAACAGACCGGTCAGACGCCGATCCCTTGGGAGGAC
GTCAAGCGCGACCTGGGTCTGACGTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2