Detailed information of TA system TA216731

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3887313..3888097
• Replicon: chromosome
• Accession: NZ_CP083530
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: K9U05_RS18195
• Protein ID: WP_000613626.1
• Coordinates: 3887313..3887807 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: K9U05_RS18200
• Protein ID: WP_139352217.1
• Coordinates: 3887804..3888097 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS18175 (3882506)	3882506..3883603	+	1098	WP_000589300.1	flagellar basal body P-ring protein FlgI	-
K9U05_RS18180 (3883603)	3883603..3884544	+	942	WP_012311956.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
K9U05_RS18185 (3884610)	3884610..3886253	+	1644	WP_228612360.1	flagellar hook-associated protein FlgK	-
K9U05_RS18190 (3886265)	3886265..3887218	+	954	WP_001212763.1	flagellar hook-associated protein FlgL	-
K9U05_RS18195 (3887313)	3887313..3887807	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
K9U05_RS18200 (3887804)	3887804..3888097	-	294	WP_139352217.1	DUF1778 domain-containing protein	Antitoxin
K9U05_RS18205 (3888231)	3888231..3891416	-	3186	WP_077516769.1	ribonuclease E	-
K9U05_RS18210 (3891989)	3891989..3892948	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T216731 WP_000613626.1 NZ_CP083530:c3887807-3887313 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T216731 NZ_CP113246:c4431368-4430781 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAATTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11139.76 Da
• Isoelectric Point: 10.5423

>AT216731 WP_139352217.1 NZ_CP083530:c3888097-3887804 [Escherichia coli]
MPAANNIPARREFLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT216731 NZ_CP113246:c4430714-4429860 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTGGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAGATGGAGCAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGGTGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCGCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGGCAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGCGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGATCTACGAATAA

Structures

Toxin

• AlphaFold DB: V0T0H9