Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3170275..3170893 Replicon chromosome
Accession NZ_CP083530
Organism Escherichia coli strain elppa2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K9U05_RS14825 Protein ID WP_001291435.1
Coordinates 3170275..3170493 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K9U05_RS14830 Protein ID WP_000344800.1
Coordinates 3170519..3170893 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U05_RS14790 (3165565) 3165565..3166137 + 573 WP_000779831.1 YbaY family lipoprotein -
K9U05_RS14795 (3166168) 3166168..3166479 - 312 WP_228612067.1 MGMT family protein -
K9U05_RS14805 (3166858) 3166858..3167211 + 354 WP_000878140.1 DUF1428 domain-containing protein -
K9U05_RS14810 (3167252) 3167252..3168802 - 1551 WP_228612069.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K9U05_RS14815 (3168966) 3168966..3169436 - 471 WP_022645280.1 YlaC family protein -
K9U05_RS14820 (3169552) 3169552..3170103 - 552 WP_000536391.1 maltose O-acetyltransferase -
K9U05_RS14825 (3170275) 3170275..3170493 - 219 WP_001291435.1 HHA domain-containing protein Toxin
K9U05_RS14830 (3170519) 3170519..3170893 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K9U05_RS14835 (3171439) 3171439..3174588 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K9U05_RS14840 (3174611) 3174611..3175804 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216728 WP_001291435.1 NZ_CP083530:c3170493-3170275 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216728 NZ_CP113246:1033621-1033899 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216728 WP_000344800.1 NZ_CP083530:c3170893-3170519 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216728 NZ_CP113246:1033911-1034216 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References