Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 2749544..2750094 Replicon chromosome
Accession NZ_CP083530
Organism Escherichia coli strain elppa2

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag K9U05_RS12840 Protein ID WP_001160966.1
Coordinates 2749780..2750094 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag K9U05_RS12835 Protein ID WP_000125568.1
Coordinates 2749544..2749777 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U05_RS12810 (2744618) 2744618..2744905 + 288 WP_000203741.1 ferredoxin-like protein FixX -
K9U05_RS12815 (2744963) 2744963..2746294 + 1332 WP_001183201.1 MFS transporter -
K9U05_RS12820 (2746402) 2746402..2746932 + 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
K9U05_RS12825 (2746925) 2746925..2748787 + 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
K9U05_RS12830 (2748979) 2748979..2749458 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
K9U05_RS12835 (2749544) 2749544..2749777 + 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
K9U05_RS12840 (2749780) 2749780..2750094 + 315 WP_001160966.1 CcdB family protein Toxin
K9U05_RS12845 (2750091) 2750091..2750939 - 849 WP_228611879.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
K9U05_RS12850 (2750946) 2750946..2751323 - 378 WP_228611881.1 Co2+/Mg2+ efflux protein ApaG -
K9U05_RS12855 (2751326) 2751326..2752147 - 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
K9U05_RS12860 (2752144) 2752144..2753133 - 990 Protein_2499 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
K9U05_RS12865 (2753133) 2753133..2754419 - 1287 WP_228611883.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T216726 WP_001160966.1 NZ_CP083530:2749780-2750094 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T216726 NZ_CP113242:223987-224484 [Vibrio sp. gvc]
ATGATGAATACGGTTCTTCTCGACAAAGACAAACACGACCGCAACCGTTTCAATTGTGGCATTGAAGCCTTAAATAATTA
CTTAAAAGTAATGTCGAGCCAGCAAGCCAAAAAAGACAATACGAGAACGTTCGTTTTGGAAGATGATAACAACAATACGC
ATATCATCGGCTTTTACACACTCACTATGACACCTATTGATTTAAAAGCCTTGCCTGACAAGTTACAGAAGAAACACCAA
TTATCAACATCAGGCGGTCTCATCGCTCGTTTAGCTGTCGATGACCGATACAAAGGTAAAGGTTTTGGCGAATGGCTGCT
TATTGATGCCCTCAGAAAATTACTCGCAGCCAGTGACAGTGTAGCGTTTCCCGTGGTTATCGTTGATGCCAAAGACGGAG
CCAAACACTTCTATGAACGTTATGGATTTCAAGCATTTCAAGACGCTGAGAACAAGCTCTTCATAACCATTGCAGATATC
CGAGCAAATCTAGGCTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT216726 WP_000125568.1 NZ_CP083530:2749544-2749777 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT216726 NZ_CP113242:223718-223990 [Vibrio sp. gvc]
ATGGCAACAACCTTACCTCGCATCACCGCCAGAGTTGATGTCGACACTCAATATTTACTCACAAAAGCTGCCGCGCTCGC
CGGCATGTCTAGCATTAATTCATTTGTTTTAAATGCGGCAATCGAAAAAGCAAAACAAGTGATTGAACGTGAACAAGCTC
TAAAGCTTAGCCAAGCTGATGCCCTATTGTTAATGGAAGCGCTCGATAATCCAGCAACAATAAACACCAAACTCAAATTA
GCATCTGAACGCTATGAGAGTAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References