Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2397384..2397881 Replicon chromosome
Accession NZ_CP083530
Organism Escherichia coli strain elppa2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K9U05_RS11270 Protein ID WP_001173343.1
Coordinates 2397708..2397881 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K9U05_RS11265 Protein ID WP_000398619.1
Coordinates 2397384..2397680 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U05_RS11245 (2394113) 2394113..2394832 - 720 WP_228613685.1 two-component system response regulator DcuR -
K9U05_RS11250 (2394829) 2394829..2396460 - 1632 WP_022646455.1 sensor histidine kinase -
K9U05_RS11255 (2396641) 2396641..2396871 + 231 WP_001683478.1 4Fe-4S mono-cluster protein YjdI -
K9U05_RS11260 (2396883) 2396883..2397155 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
K9U05_RS11265 (2397384) 2397384..2397680 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K9U05_RS11270 (2397708) 2397708..2397881 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K9U05_RS11275 (2398000) 2398000..2399517 - 1518 WP_228613687.1 lysine--tRNA ligase -
K9U05_RS11280 (2399754) 2399754..2401211 - 1458 WP_228613689.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T216724 WP_001173343.1 NZ_CP083530:2397708-2397881 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T216724 NZ_CP113242:c214056-213754 [Vibrio sp. gvc]
ATGGCTGAAATAATTTGGACGGAACCGGCTCTATCCGATCTGAATGATATTGCGGAATATATAGCCCTTGAAAATGTCAT
AGCAGCTAAACTCTTGGTACAAACGGTCTTCGCAAAGGTTGAGCGCTTAGCTGATTTTCCAGAGTCTGGACGTATTCCCT
CAGAGCTAGAGCATCTCAACTATCGTGAAGTGGTTGTTAATCCGTGTCGTATTTTCTACAGCTATGATGATAAACAGGTT
TGTATTCTTTTTGTTATGCGTGCGGAAAGGGATTTGCGTCGATTAATGCTGACTAAACAGTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT216724 WP_000398619.1 NZ_CP083530:2397384-2397680 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT216724 NZ_CP113242:c214301-214044 [Vibrio sp. gvc]
ATGAAAGTAGAACTTGTGACGTCATTGAAACGTCAAGCAACGAAGATCCTTGCTGATCTTCATCATACAAAAGAGCCAGT
ATTAATCACAGAGCATGGAAAGCCGTCAGCGTATCTTATCGACGTGGATGATTATGAGTTTATGCAAAATCGTTTAGCGA
TCCTTGAAGGTATTGCTCGTGGTGAGCGAGCGTTAGCCGATAGCAGAGTTGTTACGCATCAAAATGCTAAGGACAGAATG
TTAAAATGGCTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References