Detailed information of TA system TA216723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1668301..1668913
• Replicon: chromosome
• Accession: NZ_CP083530
• Organism: Escherichia coli strain elppa2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: K9U05_RS07855
• Protein ID: WP_000833473.1
• Coordinates: 1668728..1668913 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9U05_RS07850
• Protein ID: WP_228613370.1
• Coordinates: 1668301..1668711 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U05_RS07835 (1663758)	1663758..1664573	-	816	Protein_1531	AraC family transcriptional regulator	-
K9U05_RS07840 (1664799)	1664799..1666199	+	1401	WP_000204814.1	MFS transporter	-
K9U05_RS07845 (1666210)	1666210..1668180	+	1971	WP_077517025.1	glycoside hydrolase family 127 protein	-
K9U05_RS07850 (1668301)	1668301..1668711	-	411	WP_228613370.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K9U05_RS07855 (1668728)	1668728..1668913	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
K9U05_RS07860 (1669386)	1669386..1670465	+	1080	WP_000061477.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
K9U05_RS07865 (1670506)	1670506..1672044	-	1539	WP_000183976.1	aldehyde dehydrogenase AldB	-
K9U05_RS07870 (1672152)	1672152..1673447	-	1296	WP_000985736.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T216723 WP_000833473.1 NZ_CP083530:c1668913-1668728 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T216723 NZ_CP113242:c211593-211327 [Vibrio sp. gvc]
ATGATTAAATTTGAATTTGATGAAAACAAAAGCAGAAGTAATCTCGAAAAACATGGTATCGATTTTCATACCGCTCAAGG
ATTGTGGAATGATTCAGATCTTATCGAGATCCCTGCTAATACGAGTGATGAACCAAGATATTTGGTTGTCGGCTTACTAA
ACGGTAAGCATTGGTCAGGTGTCATTACCTACCGAGGCATGAATATCAGGCTCATCTCAGTTCGGCGTTCACGGAAAGCG
GAGATAAACCTTTATGAAAGCGCATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15244.11 Da
• Isoelectric Point: 4.4482

>AT216723 WP_228613370.1 NZ_CP083530:c1668711-1668301 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALTAHFETLCEMDEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT216723 NZ_CP113242:c211340-211095 [Vibrio sp. gvc]
ATGAAAGCGCATGAATTTGATGCTAAGTTTGAAAGTGACGACGATGACATCTTAATGGATTTGGATTTGTCGCAAGTAAA
AAGGCCAATGCATAAGCAAAAACGAGTCAATGTCGATTTTCCAGCGTGGATGCTTGAGTCTTTAGACCGAGAAGCCAATC
GTGTAGGTGTAACACGTCAATCAATCATTAAAATTTGGCTAGCAGAAAGGCTAGAAAGTGTTTCTCATCATGCAAGTGTG
AGATAA

Structures

Toxin

• AlphaFold DB: U9YXE2