Detailed information of TA system TA216709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 22778..23337
• Replicon: plasmid unnamed2
• Accession: NZ_CP083514
• Organism: Escherichia coli strain elppa4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: K9U07_RS25605
• Protein ID: WP_000421257.1
• Coordinates: 22778..23053 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A5I3EQ05
• Locus tag: K9U07_RS25610
• Protein ID: WP_001674495.1
• Coordinates: 23053..23337 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U07_RS25560 (18055)	18055..18495	-	441	Protein_27	ISNCY family transposase	-
K9U07_RS25565 (18564)	18564..18842	+	279	Protein_28	IS6 family transposase	-
K9U07_RS25570 (18838)	18838..19068	-	231	Protein_29	histidine phosphatase family protein	-
K9U07_RS25575 (19255)	19255..20235	-	981	WP_000019448.1	IS5-like element ISKpn26 family transposase	-
K9U07_RS25580 (20261)	20261..20605	+	345	WP_228587564.1	hypothetical protein	-
K9U07_RS25585 (20781)	20781..21383	+	603	WP_097468803.1	ProQ/FINO family protein	-
K9U07_RS25590 (21439)	21439..21933	+	495	WP_001459505.1	hypothetical protein	-
K9U07_RS25595 (21933)	21933..22199	+	267	WP_021576094.1	hypothetical protein	-
K9U07_RS25600 (22469)	22469..22672	-	204	Protein_35	hypothetical protein	-
K9U07_RS25605 (22778)	22778..23053	-	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9U07_RS25610 (23053)	23053..23337	-	285	WP_001674495.1	ribbon-helix-helix domain-containing protein	Antitoxin
K9U07_RS25615 (24243)	24243..25274	-	1032	WP_000907869.1	plasmid replication initiator RepA	-
K9U07_RS25620 (26232)	26232..26390	+	159	Protein_39	ash family protein	-
K9U07_RS25625 (26428)	26428..26715	+	288	WP_000074855.1	conjugal transfer protein TraA	-
K9U07_RS25630 (27157)	27157..27690	+	534	WP_001372168.1	transcription termination/antitermination NusG family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..90907	90907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T216709 WP_000421257.1 NZ_CP083514:c23053-22778 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T216709 NZ_CP113231:86088-86297 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAAAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATTGATGGTCAAGTAACGGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT216709 WP_001674495.1 NZ_CP083514:c23337-23053 [Escherichia coli]
MQMKNNSAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT216709 NZ_CP113231:85783-86076 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAAAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: A0A5I3EQ05