Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 155473..156116 Replicon plasmid unnamed1
Accession NZ_CP083513
Organism Escherichia coli strain elppa4

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag K9U07_RS25350 Protein ID WP_001034044.1
Coordinates 155473..155889 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag K9U07_RS25355 Protein ID WP_001261286.1
Coordinates 155886..156116 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U07_RS25335 (151875) 151875..152572 + 698 WP_223155668.1 IS1-like element IS1A family transposase -
K9U07_RS25340 (152826) 152826..153848 - 1023 WP_000361402.1 helicase UvrD -
K9U07_RS25345 (153833) 153833..155398 - 1566 WP_001128474.1 AAA family ATPase -
K9U07_RS25350 (155473) 155473..155889 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
K9U07_RS25355 (155886) 155886..156116 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9U07_RS25360 (156497) 156497..160291 + 3795 WP_001144731.1 hypothetical protein -
K9U07_RS25365 (160336) 160336..160752 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
K9U07_RS25370 (160749) 160749..160979 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / tet(A) / sitABCD vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA 1..171115 171115
- flank IS/Tn - - 152069..152572 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T216706 WP_001034044.1 NZ_CP083513:c155889-155473 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T216706 NZ_CP113230:3653449-3654036 [Pseudomonas aeruginosa]
ATGTCGAGGAGGGCGCCCATGTTCAAGCCGCTACCGATCACCCTGCAGCGCGGAGCCTTGCGTCTCGAGCCGCTGGTGGA
AGCGGACATTCCCGAGCTGGTGAGCCTCGCCGAAGCCAACCGCGAGGCGCTGCAGTACATGGACGGACCGACGCGGCCGG
ACTGGTACCGCCAGAGCCTCGCCGAGCAGCGCGAGGGCAGGGCGTTGCCGCTCGCCGTCCGCCTCGGCGTGCAACTGGTC
GGGACCACGCGCTTCGCCGAGTTCCTTCCCGCCTTGCCGGCCTGCGAGATCGGCTGGACCTGGCTCGACCAGGCCCAGCA
CGGCAGCGGCCTGAACCGCATGATCAAGTACCTGATGCTCAAGCATGCCTTCGACAACCTGCGCATGGTCCGCGTGCAGC
TCAGCACGGCGGCGAGCAACCTGCGCGCCCAGGGCGCCATCGACAAGCTCGGCGCGCAGCGCGAGGGCGTGCTGCGCAAC
CACCGGCGGCTGGCCGGCGGGCGGCTAGACGACACCTTCGTCTACAGCATCACCGACCACGAATGGCCGCAGGTCAAGGC
GGCGCTGGAAGCCAGCTTCACCGGCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT216706 WP_001261286.1 NZ_CP083513:c156116-155886 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT216706 NZ_CP113230:3654103-3654957 [Pseudomonas aeruginosa]
GTGCCGGACGAGACGAGCGGAGAACACACGCATTTCTGGCGCGCCGCCGAACTGGGCGGCGTCGAACTGCTGCACGCGCG
CTATGTGGAGCAGCGCTTCGCGCCGCACGTGCACAGCAGCTTCGCCCTAGTCATCATCGAGCAGGGCGCGCAGCGCTTCC
GCCATCGTGGCGGCGAGCACCTGGCCCCGCTGGGCAGCATGGTGCTGATCAACCCCGACGAAGTGCACACCGGCTCCAAG
GCCCATGATGCCGGTTGGCTCTATCGCGGCTACTACCCGGAAATGGAACAGATCGACGGGATTCTCGACGAGCTGGAAAT
CCATCATGGCGGCGTGCCGCGTTTCGCCGACAGCGTGATCCACGACCAGCCCCTGGCACGCGCCTTCGTCCACTTGCACC
GCCTGCTGGCCGAGCATGCCGGCAGCCTGGAAACCCAGACCCGCTGGCGCGAGACGATGCTCGCCCTGTTCCGTCGCCAC
GCCCGCCTGCCAGAAGCGCCGAAGGCCGGGCAGGAGCCGTTGGCGGTGGCCCGCGCCCGCGAACTGCTGGCGGCGCGGTT
GCTCGATCCGCCTTCTCTGGAGGAACTGGCGGCGGCGGTCAATCTGTCCCCGTTCCATTTTGCCCGGGTCTTCCGTCGCG
CCACCGGCATGCCGCCCCACGCCTGGCTCAGACAGCGCCGCCTGGAGCAGGCCAGGGCACTGCTCAAGAGCGGCCGGGCG
CCGCTGGAGGTGGCCCTGGAGCTGGGCTTCGCCGACCAGAGCCACCTCGGTCGCCAGTTCAAGCAGGCCTACGGGGTGGC
ACCGGGTGAATACCGCTCGGCCTGCGCGCGCTCGTCCTTGACGACATACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References