Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4349020..4349638 Replicon chromosome
Accession NZ_CP083512
Organism Escherichia coli strain elppa4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K9U07_RS21105 Protein ID WP_001291435.1
Coordinates 4349020..4349238 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K9U07_RS21110 Protein ID WP_000344800.1
Coordinates 4349264..4349638 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U07_RS21070 (4344309) 4344309..4344881 + 573 WP_000779831.1 YbaY family lipoprotein -
K9U07_RS21075 (4344912) 4344912..4345223 - 312 WP_000409911.1 MGMT family protein -
K9U07_RS21085 (4345602) 4345602..4345955 + 354 WP_000878140.1 DUF1428 domain-containing protein -
K9U07_RS21090 (4345997) 4345997..4347547 - 1551 WP_228587244.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K9U07_RS21095 (4347711) 4347711..4348181 - 471 WP_000136192.1 YlaC family protein -
K9U07_RS21100 (4348297) 4348297..4348848 - 552 WP_000102564.1 maltose O-acetyltransferase -
K9U07_RS21105 (4349020) 4349020..4349238 - 219 WP_001291435.1 HHA domain-containing protein Toxin
K9U07_RS21110 (4349264) 4349264..4349638 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K9U07_RS21115 (4350184) 4350184..4353333 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
K9U07_RS21120 (4353356) 4353356..4354549 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T216701 WP_001291435.1 NZ_CP083512:c4349238-4349020 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T216701 NZ_CP113228:c2704999-2704637 [Streptomyces albidoflavus]
ATGGACGGGGAATGGCAGATCTTCCTGGTTGACGAGGTGCGTGTTTGGCTCGCGTCCCTCGACGGGGCCGCGCACGCACG
AGTGGTACAGGCGCTGGACGTGCTGGCCGAGGAAGGGCCTGCTCTCGGAAGACCGCTTGTGGACACCTTGCGTGGTTCAG
CGGTGGCCAATTTGAAAGAGCTCCGGCCCGGCACGGTCAGAATCCTGTTCGCCTTCGATCCGTGGCGTTCCAGCATCTTG
CTGGTGGCAGGTGACAAGTCCGGGCAGCGGACTGAGTGGTACCAGGAGGCGATCCCCCTGGCCGAGCAGCGCTACGCGCT
TTACTTGAAGGAACGTGAGCGGGAAGAGGGAGGACGACCATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT216701 WP_000344800.1 NZ_CP083512:c4349638-4349264 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT216701 NZ_CP113228:c2704640-2704323 [Streptomyces albidoflavus]
ATGACGAGTTACACCCGTTGGCAGGACATTCGTCCCGAGCATGTCGCCCGCGCAGGGGGTGAGGAAGCCGTACGCGCGGG
TAAGGAGGAGCTGCTCGCGCAGGTCACCGGGAGGAAGCTCGCGGAGCTGCGTCGCGCCCGAGGGCTGACCCAGCAGGAGG
TGGCGGACCGGATGGGGGTCAGCAAGGGGCGGATCTCCCAGATCGAGCGGGGGCACCTTTCGGGACAGGAAGTGCTGGCC
CGATTCGCGACCGCTCTCGGAGGCCGTCTCCATCAGGCGATCTACTTCGACGACGGCGACATCGCCACCATCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References