Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3738403..3738979 Replicon chromosome
Accession NZ_CP083512
Organism Escherichia coli strain elppa4

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag K9U07_RS18120 Protein ID WP_001297643.1
Coordinates 3738403..3738690 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag K9U07_RS18125 Protein ID WP_000063156.1
Coordinates 3738677..3738979 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U07_RS18100 (3734656) 3734656..3735936 - 1281 WP_001037380.1 DUF445 domain-containing protein -
K9U07_RS18105 (3735977) 3735977..3737209 - 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
K9U07_RS18110 (3737355) 3737355..3737450 - 96 Protein_3535 dienelactone hydrolase family protein -
K9U07_RS18115 (3737507) 3737507..3738204 + 698 Protein_3536 IS1 family transposase -
K9U07_RS18120 (3738403) 3738403..3738690 + 288 WP_001297643.1 BrnT family toxin Toxin
K9U07_RS18125 (3738677) 3738677..3738979 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
K9U07_RS18130 (3739013) 3739013..3739969 - 957 WP_228587189.1 GTPase -
K9U07_RS18135 (3739980) 3739980..3740183 - 204 WP_000467859.1 YbdD/YjiX family protein -
K9U07_RS18140 (3740233) 3740233..3742383 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T216697 WP_001297643.1 NZ_CP083512:3738403-3738690 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T216697 NZ_CP113217:c60650-60180 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT216697 WP_000063156.1 NZ_CP083512:3738677-3738979 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT216697 NZ_CP113217:c61090-60647 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References