Detailed information of TA system TA216696

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3665497..3666329
• Replicon: chromosome
• Accession: NZ_CP083512
• Organism: Escherichia coli strain elppa4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: K9U07_RS17850
• Protein ID: WP_000854765.1
• Coordinates: 3665955..3666329 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: K9U07_RS17845
• Protein ID: WP_001295723.1
• Coordinates: 3665497..3665865 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U07_RS17815 (3662615)	3662615..3662810	+	196	Protein_3476	DUF905 family protein	-
K9U07_RS17820 (3662928)	3662928..3663746	+	819	WP_001234716.1	DUF932 domain-containing protein	-
K9U07_RS17825 (3663746)	3663746..3663919	+	174	WP_001183321.1	hypothetical protein	-
K9U07_RS17830 (3664085)	3664085..3664558	+	474	WP_001387789.1	antirestriction protein	-
K9U07_RS17835 (3664574)	3664574..3665050	+	477	WP_001186773.1	RadC family protein	-
K9U07_RS17840 (3665113)	3665113..3665334	+	222	WP_000692300.1	DUF987 domain-containing protein	-
K9U07_RS17845 (3665497)	3665497..3665865	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K9U07_RS17850 (3665955)	3665955..3666329	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
K9U07_RS17855 (3666326)	3666326..3666817	+	492	WP_001586604.1	DUF5983 family protein	-
K9U07_RS17860 (3666834)	3666834..3667010	+	177	WP_001586605.1	DUF957 domain-containing protein	-
K9U07_RS17865 (3667116)	3667116..3667265	+	150	Protein_3486	hypothetical protein	-
K9U07_RS17870 (3668269)	3668269..3670305	+	2037	Protein_3487	hypothetical protein	-
K9U07_RS17875 (3670704)	3670704..3670883	-	180	Protein_3488	peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T216696 WP_000854765.1 NZ_CP083512:3665955-3666329 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T216696 NZ_CP113216:c5352313-5351930 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT216696 WP_001295723.1 NZ_CP083512:3665497-3665865 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT216696 NZ_CP113216:c5352555-5352313 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTACTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52