Detailed information of TA system TA216694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3572888..3573483
• Replicon: chromosome
• Accession: NZ_CP083512
• Organism: Escherichia coli strain elppa4

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: K9U07_RS17360
• Protein ID: WP_000239581.1
• Coordinates: 3573133..3573483 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: K9U07_RS17355
• Protein ID: WP_001223213.1
• Coordinates: 3572888..3573139 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U07_RS17345 (3568553)	3568553..3572332	+	3780	WP_228587181.1	autotransporter assembly complex protein TamB	-
K9U07_RS17350 (3572335)	3572335..3572676	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
K9U07_RS17355 (3572888)	3572888..3573139	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
K9U07_RS17360 (3573133)	3573133..3573483	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
K9U07_RS17365 (3573562)	3573562..3574092	-	531	WP_000055075.1	inorganic diphosphatase	-
K9U07_RS17370 (3574402)	3574402..3575358	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
K9U07_RS17375 (3575498)	3575498..3577000	+	1503	WP_228587183.1	sugar ABC transporter ATP-binding protein	-
K9U07_RS17380 (3577014)	3577014..3578036	+	1023	WP_001586590.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T216694 WP_000239581.1 NZ_CP083512:3573133-3573483 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T216694 NZ_CP113216:c4862584-4862300 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGTGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT216694 WP_001223213.1 NZ_CP083512:3572888-3573139 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT216694 NZ_CP113216:c4862816-4862574 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGTGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7