Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3470932..3471429 Replicon chromosome
Accession NZ_CP083512
Organism Escherichia coli strain elppa4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K9U07_RS16840 Protein ID WP_001173343.1
Coordinates 3471256..3471429 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2X1N3L1
Locus tag K9U07_RS16835 Protein ID WP_001560287.1
Coordinates 3470932..3471228 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U07_RS16815 (3467661) 3467661..3468380 - 720 WP_000611288.1 two-component system response regulator DcuR -
K9U07_RS16820 (3468377) 3468377..3470008 - 1632 WP_001216477.1 sensor histidine kinase -
K9U07_RS16825 (3470189) 3470189..3470419 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
K9U07_RS16830 (3470431) 3470431..3470703 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
K9U07_RS16835 (3470932) 3470932..3471228 + 297 WP_001560287.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K9U07_RS16840 (3471256) 3471256..3471429 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K9U07_RS16845 (3471548) 3471548..3473065 - 1518 WP_000003806.1 lysine--tRNA ligase -
K9U07_RS16850 (3473302) 3473302..3474759 - 1458 WP_000856819.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T216693 WP_001173343.1 NZ_CP083512:3471256-3471429 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T216693 NZ_CP113216:c4779540-4779199 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCATGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11441.94 Da        Isoelectric Point: 4.2924

>AT216693 WP_001560287.1 NZ_CP083512:3470932-3471228 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMHDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT216693 NZ_CP113216:c4779902-4779561 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTCAGCGAAGCAGAATGTC
TTAAGCTGGATGTGGCATTCCCACATTTTATCAGCCAGATGGAGTCGATGTTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTGTGTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1N3L1

References