Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 2768803..2769415 Replicon chromosome
Accession NZ_CP083512
Organism Escherichia coli strain elppa4

Toxin (Protein)


Gene name hicA Uniprot ID A0A167AMN3
Locus tag K9U07_RS13490 Protein ID WP_000833474.1
Coordinates 2769230..2769415 (-) Length 62 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1V3VHB3
Locus tag K9U07_RS13485 Protein ID WP_000499746.1
Coordinates 2768803..2769213 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U07_RS13470 (2764315) 2764315..2765130 - 816 WP_000891838.1 AraC family transcriptional regulator -
K9U07_RS13475 (2765356) 2765356..2766756 + 1401 WP_000204815.1 MFS transporter -
K9U07_RS13480 (2766767) 2766767..2768731 + 1965 WP_001026871.1 glycoside hydrolase family 127 protein -
K9U07_RS13485 (2768803) 2768803..2769213 - 411 WP_000499746.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K9U07_RS13490 (2769230) 2769230..2769415 - 186 WP_000833474.1 type II toxin-antitoxin system HicA family toxin Toxin
K9U07_RS13495 (2769888) 2769888..2770967 + 1080 WP_000061479.1 type I restriction enzyme HsdR N-terminal domain-containing protein -
K9U07_RS13500 (2771008) 2771008..2772546 - 1539 WP_001469350.1 aldehyde dehydrogenase AldB -
K9U07_RS13505 (2772747) 2772747..2773898 - 1152 WP_000741506.1 L-threonine dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-129)


Sequences

Toxin        


Download         Length: 62 a.a.        Molecular weight: 6819.94 Da        Isoelectric Point: 12.0345

>T216692 WP_000833474.1 NZ_CP083512:c2769415-2769230 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSRHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Download         Length: 186 bp

>T216692 NZ_CP113216:c4739939-4739406 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATAAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 15290.22 Da        Isoelectric Point: 4.5486

>AT216692 WP_000499746.1 NZ_CP083512:c2769213-2768803 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDYAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Download         Length: 411 bp

>AT216692 NZ_CP113216:c4740216-4739950 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A167AMN3

Antitoxin

Source ID Structure
AlphaFold DB A0A1V3VHB3

References