Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 362508..363049 Replicon plasmid pRHL1
Accession NC_008269
Organism Rhodococcus jostii RHA1

Toxin (Protein)


Gene name mazF Uniprot ID Q0RZ55
Locus tag RHA1_RS37425 Protein ID WP_007298577.1
Coordinates 362508..362807 (-) Length 100 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2S8J1A3
Locus tag RHA1_RS37430 Protein ID WP_039951845.1
Coordinates 362807..363049 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RHA1_RS37410 358042..358467 + 426 WP_011599235.1 nuclear transport factor 2 family protein -
RHA1_RS37415 358464..359345 + 882 WP_007298579.1 SDR family oxidoreductase -
RHA1_RS37420 359891..362251 - 2361 WP_007298578.1 helicase-associated domain-containing protein -
RHA1_RS37425 362508..362807 - 300 WP_007298577.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
RHA1_RS37430 362807..363049 - 243 WP_039951845.1 hypothetical protein Antitoxin
RHA1_RS37435 363260..363962 - 703 Protein_362 IS6 family transposase -
RHA1_RS37440 364036..364926 - 891 WP_011599239.1 class I SAM-dependent methyltransferase -
RHA1_RS37445 365679..365858 - 180 Protein_364 hypothetical protein -
RHA1_RS37450 366020..366880 + 861 WP_007298572.1 GNAT family N-acetyltransferase -
RHA1_RS37455 366877..367866 - 990 WP_007298571.1 IS481-like element ISRhosp6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..1123075 1123075
- flank IS/Tn - - 366877..367866 989


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-98)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 10545.30 Da        Isoelectric Point: 9.4104

>T21668 WP_007298577.1 NC_008269:c362807-362508 [Rhodococcus jostii RHA1]
MRPIHAAKLDKTRPVLVLTRELVRPHLSRVTVAPITGTVRGLSTEVPVGPANGLEKESVVSCDNIVTIPVSALGRGLGFF
FPSQEAALTTAIRAAFDLE

Download         Length: 300 bp

>T21668 NC_008269:c362807-362508 [Rhodococcus jostii RHA1]
ATGCGGCCGATTCACGCCGCGAAGCTGGACAAGACGCGGCCGGTGCTGGTGTTGACCCGGGAGTTGGTGCGCCCGCACCT
GAGCCGGGTGACGGTGGCGCCGATCACCGGCACCGTGCGGGGACTGTCCACCGAGGTGCCGGTGGGGCCGGCCAACGGGC
TGGAGAAGGAGTCGGTGGTCAGTTGCGACAACATCGTCACGATTCCGGTGTCGGCGTTGGGGCGCGGTCTCGGGTTCTTC
TTTCCGTCGCAGGAGGCTGCCCTGACCACAGCGATCCGGGCCGCGTTCGACCTGGAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8818.85 Da        Isoelectric Point: 5.1224

>AT21668 WP_039951845.1 NC_008269:c363049-362807 [Rhodococcus jostii RHA1]
MSTQIAVRLPDEIVAFVDGEVRDHRAPSRAALVLRALERERRRQVAARDAEILSRARGGDDPDGLDDLARHAAGLFSDLD

Download         Length: 243 bp

>AT21668 NC_008269:c363049-362807 [Rhodococcus jostii RHA1]
ATGAGTACACAGATTGCGGTGCGGTTGCCGGATGAGATTGTGGCCTTCGTCGACGGTGAGGTGCGCGACCATCGGGCGCC
CAGCCGGGCCGCCCTGGTGTTGCGGGCGCTCGAGCGGGAGCGGCGTCGACAGGTCGCCGCCCGGGATGCCGAGATTCTGT
CCCGGGCGCGCGGCGGGGACGACCCGGACGGCCTCGATGACCTCGCCCGGCATGCCGCGGGTCTGTTCAGCGATCTCGAC
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q0RZ55

Antitoxin

Source ID Structure
AlphaFold DB A0A2S8J1A3

References