Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3550044..3550675 Replicon chromosome
Accession NC_008258
Organism Shigella flexneri 5 str. 8401

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag SFV_RS19165 Protein ID WP_001259385.1
Coordinates 3550400..3550675 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag SFV_RS19160 Protein ID WP_000593555.1
Coordinates 3550044..3550403 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SFV_RS19135 3546176..3547120 + 945 WP_000947077.1 nickel ABC transporter permease subunit NikB -
SFV_RS19140 3547117..3547950 + 834 WP_001008974.1 nickel ABC transporter permease subunit NikC -
SFV_RS19145 3547950..3548714 + 765 WP_001136248.1 nickel import ATP-binding protein NikD -
SFV_RS19150 3548711..3549517 + 807 WP_000173670.1 nickel import ATP-binding protein NikE -
SFV_RS19155 3549523..3549924 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
SFV_RS19160 3550044..3550403 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
SFV_RS19165 3550400..3550675 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
SFV_RS19170 3550734..3551858 - 1125 WP_001216257.1 ABC transporter permease -
SFV_RS19175 3551858..3554593 - 2736 WP_000149171.1 ribosome-associated ATPase/putative transporter RbbA -
SFV_RS19180 3554590..3555657 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T21667 WP_001259385.1 NC_008258:c3550675-3550400 [Shigella flexneri 5 str. 8401]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T21667 NC_008258:c3550675-3550400 [Shigella flexneri 5 str. 8401]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT21667 WP_000593555.1 NC_008258:c3550403-3550044 [Shigella flexneri 5 str. 8401]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT21667 NC_008258:c3550403-3550044 [Shigella flexneri 5 str. 8401]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCAGCTGTTATTACCTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGAG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References