Detailed information of TA system TA216668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 5065911..5066616
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: K9U08_RS24475
• Protein ID: WP_000539521.1
• Coordinates: 5066230..5066616 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K9U08_RS24470
• Protein ID: WP_001280945.1
• Coordinates: 5065911..5066240 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS24455 (5061068)	5061068..5061979	+	912	WP_001236019.1	glutathione ABC transporter permease GsiD	-
K9U08_RS24460 (5062157)	5062157..5064505	+	2349	WP_021523051.1	EAL domain-containing protein	-
K9U08_RS24465 (5064513)	5064513..5065841	+	1329	WP_000086873.1	GGDEF domain-containing protein	-
K9U08_RS24470 (5065911)	5065911..5066240	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
K9U08_RS24475 (5066230)	5066230..5066616	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K9U08_RS24480 (5066842)	5066842..5068167	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
K9U08_RS24485 (5068380)	5068380..5068763	+	384	WP_000497137.1	biofilm formation regulator BssR	-
K9U08_RS24490 (5068874)	5068874..5069989	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
K9U08_RS24495 (5069986)	5069986..5070612	-	627	WP_020233670.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T216668 WP_000539521.1 NZ_CP083503:c5066616-5066230 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T216668 NZ_CP113204:54754-55236 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT216668 WP_001280945.1 NZ_CP083503:c5066240-5065911 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT216668 NZ_CP113204:54500-54766 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6