Detailed information of TA system TA216666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4440341..4442030
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A829L165
• Locus tag: K9U08_RS21645
• Protein ID: WP_001521912.1
• Coordinates: 4441110..4442030 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: K9U08_RS21640
• Protein ID: WP_001521911.1
• Coordinates: 4440341..4441123 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS21620 (4435650)	4435650..4435808	-	159	Protein_4218	integrase	-
K9U08_RS21625 (4435900)	4435900..4436002	-	103	Protein_4219	helix-turn-helix domain-containing protein	-
K9U08_RS21630 (4435999)	4435999..4436469	-	471	WP_000201185.1	hypothetical protein	-
K9U08_RS21635 (4436816)	4436816..4440025	-	3210	WP_048237838.1	RNA-directed DNA polymerase	-
K9U08_RS21640 (4440341)	4440341..4441123	+	783	WP_001521911.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
K9U08_RS21645 (4441110)	4441110..4442030	+	921	WP_001521912.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
K9U08_RS21650 (4442464)	4442464..4443348	-	885	WP_001119037.1	LysR family transcriptional regulator	-
K9U08_RS21655 (4443613)	4443613..4444671	+	1059	WP_122452191.1	alpha/beta hydrolase	-
K9U08_RS21660 (4444813)	4444813..4445940	+	1128	WP_001521913.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34601.04 Da
• Isoelectric Point: 7.2649

>T216666 WP_001521912.1 NZ_CP083503:4441110-4442030 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T216666 NZ_CP113202:c5176531-5176148 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29168.46 Da
• Isoelectric Point: 8.4458

>AT216666 WP_001521911.1 NZ_CP083503:4440341-4441123 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELVAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQFIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT216666 NZ_CP113202:c5176773-5176531 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A829L165