Detailed information of TA system TA216664

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4030630..4031444
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: K9U08_RS19720
• Protein ID: WP_001054376.1
• Coordinates: 4031187..4031444 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: K9U08_RS19715
• Protein ID: WP_001309181.1
• Coordinates: 4030630..4031175 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS19685 (4026321)	4026321..4027634	-	1314	WP_228588429.1	PTS sugar transporter subunit IIC SgcC	-
K9U08_RS19690 (4027646)	4027646..4027924	-	279	WP_000722973.1	PTS sugar transporter subunit IIB SgcB	-
K9U08_RS19695 (4027921)	4027921..4029042	-	1122	WP_000010829.1	M42 family metallopeptidase	-
K9U08_RS19700 (4029287)	4029287..4029403	-	117	Protein_3845	VOC family protein	-
K9U08_RS19705 (4029441)	4029441..4029659	-	219	Protein_3846	hypothetical protein	-
K9U08_RS19710 (4029828)	4029828..4030574	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
K9U08_RS19715 (4030630)	4030630..4031175	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
K9U08_RS19720 (4031187)	4031187..4031444	-	258	WP_001054376.1	YjhX family toxin	Toxin
K9U08_RS19725 (4031935)	4031935..4032066	-	132	WP_001309182.1	hypothetical protein	-
K9U08_RS19730 (4032330)	4032330..4033922	-	1593	WP_048237919.1	IS66 family transposase	-
K9U08_RS19735 (4033953)	4033953..4034303	-	351	WP_000624718.1	IS66 family insertion sequence element accessory protein TnpB	-
K9U08_RS19740 (4034300)	4034300..4034725	-	426	WP_000422741.1	transposase	-
K9U08_RS19745 (4034812)	4034812..4035941	+	1130	Protein_3854	helicase YjhR	-
K9U08_RS19750 (4036209)	4036209..4036414	-	206	Protein_3855	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T216664 WP_001054376.1 NZ_CP083503:c4031444-4031187 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T216664 NZ_CP113202:c4677989-4677705 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT216664 WP_001309181.1 NZ_CP083503:c4031175-4030630 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT216664 NZ_CP113202:c4678221-4677979 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8