Detailed information of TA system TA216662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3906037..3906632
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: K9U08_RS19080
• Protein ID: WP_000239581.1
• Coordinates: 3906282..3906632 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: K9U08_RS19075
• Protein ID: WP_001223213.1
• Coordinates: 3906037..3906288 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS19065 (3901702)	3901702..3905481	+	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-
K9U08_RS19070 (3905484)	3905484..3905825	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
K9U08_RS19075 (3906037)	3906037..3906288	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
K9U08_RS19080 (3906282)	3906282..3906632	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
K9U08_RS19085 (3906711)	3906711..3907241	-	531	WP_000055075.1	inorganic diphosphatase	-
K9U08_RS19090 (3907551)	3907551..3908507	+	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
K9U08_RS19095 (3908640)	3908640..3910142	+	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
K9U08_RS19100 (3910156)	3910156..3911178	+	1023	WP_001361374.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T216662 WP_000239581.1 NZ_CP083503:3906282-3906632 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T216662 NZ_CP113202:3964552-3964770 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT216662 WP_001223213.1 NZ_CP083503:3906037-3906288 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT216662 NZ_CP113202:3964151-3964525 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7