Detailed information of TA system TA216656

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 2389756..2390410
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: F4T2L4
• Locus tag: K9U08_RS11860
• Protein ID: WP_000244765.1
• Coordinates: 2389756..2390163 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: S1PPB1
• Locus tag: K9U08_RS11865
• Protein ID: WP_000354046.1
• Coordinates: 2390144..2390410 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS11840 (2385713)	2385713..2387446	-	1734	WP_000813190.1	single-stranded-DNA-specific exonuclease RecJ	-
K9U08_RS11845 (2387452)	2387452..2388162	-	711	WP_000715230.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
K9U08_RS11850 (2388187)	2388187..2389083	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
K9U08_RS11855 (2389195)	2389195..2389716	+	522	WP_001055874.1	flavodoxin FldB	-
K9U08_RS11860 (2389756)	2389756..2390163	-	408	WP_000244765.1	protein YgfX	Toxin
K9U08_RS11865 (2390144)	2390144..2390410	-	267	WP_000354046.1	FAD assembly factor SdhE	Antitoxin
K9U08_RS11870 (2390653)	2390653..2391633	+	981	WP_049038218.1	tRNA-modifying protein YgfZ	-
K9U08_RS11875 (2391710)	2391710..2392369	-	660	WP_000250274.1	hemolysin III family protein	-
K9U08_RS11880 (2392533)	2392533..2392844	-	312	WP_001182956.1	N(4)-acetylcytidine aminohydrolase	-
K9U08_RS11885 (2392889)	2392889..2394322	+	1434	WP_063082439.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16043.95 Da
• Isoelectric Point: 11.5202

>T216656 WP_000244765.1 NZ_CP083503:c2390163-2389756 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T216656 NZ_CP113200:c41143-40673 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10547.12 Da
• Isoelectric Point: 5.1599

>AT216656 WP_000354046.1 NZ_CP083503:c2390410-2390144 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT216656 NZ_CP113200:c41583-41140 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A454A7D7

Antitoxin

• AlphaFold DB: A0A7U9QD57