Detailed information of TA system TA216642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 682530..683168
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: K9U08_RS03470
• Protein ID: WP_001306887.1
• Coordinates: 682530..682706 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9U08_RS03475
• Protein ID: WP_001270286.1
• Coordinates: 682752..683168 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS03445 (677586)	677586..678044	+	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-
K9U08_RS03450 (678152)	678152..679363	-	1212	WP_023143671.1	BenE family transporter YdcO	-
K9U08_RS03455 (679416)	679416..679952	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
K9U08_RS03460 (680025)	680025..681986	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K9U08_RS03465 (682078)	682078..682308	-	231	WP_000494241.1	YncJ family protein	-
K9U08_RS03470 (682530)	682530..682706	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K9U08_RS03475 (682752)	682752..683168	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K9U08_RS03480 (683247)	683247..684653	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
K9U08_RS03485 (684898)	684898..686043	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
K9U08_RS03490 (686061)	686061..687074	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
K9U08_RS03495 (687075)	687075..688016	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	677586..678044	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T216642 WP_001306887.1 NZ_CP083503:682530-682706 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T216642 NZ_CP113193:182401-182682 [Klebsiella pneumoniae]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCCTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT216642 WP_001270286.1 NZ_CP083503:682752-683168 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT216642 NZ_CP113193:182160-182411 [Klebsiella pneumoniae]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76