Detailed information of TA system TA216634

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 198019..198863
• Replicon: chromosome
• Accession: NZ_CP083503
• Organism: Escherichia coli strain elppa5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: K9U08_RS00880
• Protein ID: WP_000854686.1
• Coordinates: 198480..198863 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: K9U08_RS00875
• Protein ID: WP_001285602.1
• Coordinates: 198019..198399 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U08_RS00840 (194268)	194268..194723	+	456	WP_001504120.1	IrmA family protein	-
K9U08_RS00845 (194802)	194802..195035	+	234	WP_001119719.1	DUF905 family protein	-
K9U08_RS00850 (195135)	195135..195953	+	819	WP_001234732.1	DUF932 domain-containing protein	-
K9U08_RS00855 (196045)	196045..196530	+	486	WP_000214307.1	antirestriction protein	-
K9U08_RS00860 (196546)	196546..197022	+	477	WP_001186726.1	RadC family protein	-
K9U08_RS00865 (197085)	197085..197306	+	222	WP_000692298.1	DUF987 domain-containing protein	-
K9U08_RS00870 (197325)	197325..198008	+	684	WP_000086769.1	hypothetical protein	-
K9U08_RS00875 (198019)	198019..198399	+	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K9U08_RS00880 (198480)	198480..198863	+	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
K9U08_RS00885 (198860)	198860..199348	+	489	WP_001054233.1	DUF5983 family protein	-
K9U08_RS00890 (199365)	199365..199562	+	198	WP_000839253.1	DUF957 domain-containing protein	-
K9U08_RS00895 (199647)	199647..200489	+	843	WP_001274547.1	DUF4942 domain-containing protein	-
K9U08_RS00905 (200960)	200960..201898	+	939	WP_001522839.1	glyoxylate/hydroxypyruvate reductase GhrA	-
K9U08_RS00910 (201953)	201953..202690	+	738	WP_000283664.1	zinc-binding phosphatase	-
K9U08_RS00915 (202714)	202714..203268	+	555	WP_001001921.1	molecular chaperone YcdY	-
K9U08_RS00920 (203370)	203370..203861	+	492	WP_001308552.1	DUF1097 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T216634 WP_000854686.1 NZ_CP083503:198480-198863 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T216634 NZ_CP113192:2005760-2005862 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT216634 WP_001285602.1 NZ_CP083503:198019-198399 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT216634 NZ_CP113192:c2005869-2005724 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT