Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 118238..118881 Replicon plasmid unnamed2
Accession NZ_CP083499
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag K9U09_RS24085 Protein ID WP_001044768.1
Coordinates 118238..118654 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag K9U09_RS24090 Protein ID WP_001261287.1
Coordinates 118651..118881 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS24070 (114737) 114737..115327 - 591 WP_000194575.1 hypothetical protein -
K9U09_RS24075 (115327) 115327..115584 - 258 WP_000343085.1 hypothetical protein -
K9U09_RS24080 (115938) 115938..118076 + 2139 WP_000350638.1 AAA family ATPase -
K9U09_RS24085 (118238) 118238..118654 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
K9U09_RS24090 (118651) 118651..118881 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9U09_RS24095 (119177) 119177..119467 + 291 WP_000111771.1 hypothetical protein -
K9U09_RS24100 (119457) 119457..120356 + 900 WP_000963206.1 nucleotide-binding protein -
K9U09_RS24105 (120406) 120406..122631 - 2226 WP_000698737.1 P-loop NTPase fold protein -
K9U09_RS24110 (122633) 122633..123721 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCMY-2 / sul3 / ant(3'')-Ia / floR / lnu(F) / aadA2 / aac(3)-IId - 1..130873 130873


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T216632 WP_001044768.1 NZ_CP083499:c118654-118238 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T216632 NZ_CP113192:c792778-792401 [Klebsiella pneumoniae]
ATGAAAACATTACCCGACACACACGAACGGGAGGCGTCACGCTGCTCGTCTCCTGTGACAATCTGGCAGACACTCCTGGC
CCGGCTGCTGGGCCAGCATTACAGCCTCACGCTGAATGACACGCCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAGTACGCGCTGGTACGTACCGACCAGCCGGGATTC
GGCACCTGTGCCCATTCGCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGTTGACCCGCAG
CAACTACAGAACGGTGAACGACATTATCCGGGGTAAAGATGCGGAGGCAAAACAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT216632 WP_001261287.1 NZ_CP083499:c118881-118651 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT216632 NZ_CP113192:c793202-792825 [Klebsiella pneumoniae]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAGGGTAACCGGTTGCATTACCTTGCAGATCGCGCAGGTATCAGAGGTCAGT
TCAGCAACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGCTGAAACAACTGGAGTTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
GGGTTACGTTTATATGGCTGTTTATCCGACACCGGCAGCTCCCGCAACAACCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References