Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 112601..113126 Replicon plasmid unnamed1
Accession NZ_CP083498
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag K9U09_RS23340 Protein ID WP_001159868.1
Coordinates 112821..113126 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag K9U09_RS23335 Protein ID WP_000813634.1
Coordinates 112601..112819 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS23320 (111011) 111011..111427 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
K9U09_RS23325 (111424) 111424..111654 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -
K9U09_RS23330 (111919) 111919..112311 + 393 WP_228596861.1 HEPN family nuclease -
K9U09_RS23335 (112601) 112601..112819 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
K9U09_RS23340 (112821) 112821..113126 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
K9U09_RS23345 (113127) 113127..113933 + 807 WP_000016982.1 site-specific integrase -
K9U09_RS23350 (114689) 114689..115444 + 756 WP_000852146.1 replication initiation protein RepE -
K9U09_RS23355 (116032) 116032..117198 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / sitABCD senB / iucA / iucB / iucC / iucD / iutA 1..128175 128175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T216627 WP_001159868.1 NZ_CP083498:112821-113126 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T216627 NZ_CP113191:c4475487-4475146 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCTATAATTTCGCGGGCGGCGAAGCCCTGTCTGTCGCCCGTTGCAGTCTGGCAAATGCTACTTAC
ACGCCTGCTGGAAAAACACTATGGTCTGACCCTGAACGATACGCCGTTCTGTGATGAAACCGTTATACAGGAACATATCG
ATGCCGGTATTACGCTGGCTGATGCCGTTAATTTTCTGGTGGAAAAATACGGACTGGTTCGTATCGACAGGAATGGATTT
GTCTGGCAGGAACAATCACCTTATCTTCGGGCCGTTGATATTCTGCGAGCGCGGCAGGCAACTGGCTTATTGCGACAAAG
CCGTAACAACGCAATACGTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT216627 WP_000813634.1 NZ_CP083498:112601-112819 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT216627 NZ_CP113191:c4475825-4475508 [Klebsiella pneumoniae]
ATGAGCAACATTACATGGGGCCTGCAGCGGGATACCACGCCGCGTCTGGGAGCCCGTCTGGTACAGGAGGGGAACCAACT
GCATTATCTGGCTGACCGGGCCAGTATGACCGGTAAATTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCAGCCAGATGGAATCGATGCTGATCACTGGTGAAATGAATCCTCGCCATGCCCACTGTGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGCCGTTTACCCCATCCAACGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References