Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 111011..111654 Replicon plasmid unnamed1
Accession NZ_CP083498
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag K9U09_RS23320 Protein ID WP_001034046.1
Coordinates 111011..111427 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag K9U09_RS23325 Protein ID WP_001261278.1
Coordinates 111424..111654 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS23305 (106148) 106148..106564 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
K9U09_RS23310 (106561) 106561..106791 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
K9U09_RS23315 (107172) 107172..110966 + 3795 WP_001144732.1 hypothetical protein -
K9U09_RS23320 (111011) 111011..111427 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
K9U09_RS23325 (111424) 111424..111654 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9U09_RS23330 (111919) 111919..112311 + 393 WP_228596861.1 HEPN family nuclease -
K9U09_RS23335 (112601) 112601..112819 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
K9U09_RS23340 (112821) 112821..113126 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
K9U09_RS23345 (113127) 113127..113933 + 807 WP_000016982.1 site-specific integrase -
K9U09_RS23350 (114689) 114689..115444 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / sitABCD senB / iucA / iucB / iucC / iucD / iutA 1..128175 128175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T216626 WP_001034046.1 NZ_CP083498:c111427-111011 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T216626 NZ_CP113191:c4328941-4328531 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT216626 WP_001261278.1 NZ_CP083498:c111654-111424 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT216626 NZ_CP113191:c4329188-4328922 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References