Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 106148..106791 Replicon plasmid unnamed1
Accession NZ_CP083498
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag K9U09_RS23305 Protein ID WP_001034044.1
Coordinates 106148..106564 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag K9U09_RS23310 Protein ID WP_001261286.1
Coordinates 106561..106791 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS23290 (102553) 102553..103247 + 695 WP_228596859.1 IS1-like element IS1A family transposase -
K9U09_RS23295 (103501) 103501..104523 - 1023 WP_000361402.1 helicase UvrD -
K9U09_RS23300 (104508) 104508..106073 - 1566 WP_001128474.1 AAA family ATPase -
K9U09_RS23305 (106148) 106148..106564 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
K9U09_RS23310 (106561) 106561..106791 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K9U09_RS23315 (107172) 107172..110966 + 3795 WP_001144732.1 hypothetical protein -
K9U09_RS23320 (111011) 111011..111427 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
K9U09_RS23325 (111424) 111424..111654 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5 / sitABCD senB / iucA / iucB / iucC / iucD / iutA 1..128175 128175
- flank IS/Tn - - 102747..103247 500


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T216625 WP_001034044.1 NZ_CP083498:c106564-106148 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T216625 NZ_CP113191:c3260433-3260331 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT216625 WP_001261286.1 NZ_CP083498:c106791-106561 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT216625 NZ_CP113191:3260324-3260469 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References