Detailed information of TA system TA216620

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4364702..4365327
• Replicon: chromosome
• Accession: NZ_CP083497
• Organism: Escherichia coli strain elppa6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K9U09_RS20995
• Protein ID: WP_000911330.1
• Coordinates: 4364929..4365327 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: K9U09_RS20990
• Protein ID: WP_000450524.1
• Coordinates: 4364702..4364929 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U09_RS20965 (4360505)	4360505..4360975	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
K9U09_RS20970 (4360975)	4360975..4361547	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
K9U09_RS20975 (4361693)	4361693..4362571	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
K9U09_RS20980 (4362588)	4362588..4363622	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
K9U09_RS20985 (4363835)	4363835..4364548	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
K9U09_RS20990 (4364702)	4364702..4364929	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K9U09_RS20995 (4364929)	4364929..4365327	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
K9U09_RS21000 (4365474)	4365474..4366337	+	864	WP_228596659.1	neutral zinc metallopeptidase	-
K9U09_RS21005 (4366352)	4366352..4368367	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
K9U09_RS21010 (4368441)	4368441..4369139	+	699	WP_000679823.1	esterase	-
K9U09_RS21015 (4369249)	4369249..4369449	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T216620 WP_000911330.1 NZ_CP083497:4364929-4365327 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T216620 NZ_CP113191:397012-397296 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT216620 WP_000450524.1 NZ_CP083497:4364702-4364929 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT216620 NZ_CP113191:396780-397022 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33