Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3940177..3940831 Replicon chromosome
Accession NZ_CP083497
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag K9U09_RS18975 Protein ID WP_000244772.1
Coordinates 3940424..3940831 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K9U09_RS18970 Protein ID WP_000354046.1
Coordinates 3940177..3940443 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS18945 (3935465) 3935465..3936208 + 744 WP_000951964.1 SDR family oxidoreductase -
K9U09_RS18950 (3936265) 3936265..3937698 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
K9U09_RS18955 (3937743) 3937743..3938054 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
K9U09_RS18960 (3938218) 3938218..3938877 + 660 WP_000250274.1 hemolysin III family protein -
K9U09_RS18965 (3938954) 3938954..3939934 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K9U09_RS18970 (3940177) 3940177..3940443 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K9U09_RS18975 (3940424) 3940424..3940831 + 408 WP_000244772.1 protein YgfX Toxin
K9U09_RS18980 (3940871) 3940871..3941392 - 522 WP_001055874.1 flavodoxin FldB -
K9U09_RS18985 (3941504) 3941504..3942400 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K9U09_RS18990 (3942425) 3942425..3943135 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K9U09_RS18995 (3943141) 3943141..3944874 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T216618 WP_000244772.1 NZ_CP083497:3940424-3940831 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216618 NZ_CP113189:c6172-5867 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216618 WP_000354046.1 NZ_CP083497:3940177-3940443 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216618 NZ_CP113189:c6392-6174 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References