Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3344556..3345142 Replicon chromosome
Accession NZ_CP083497
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag K9U09_RS15965 Protein ID WP_042014162.1
Coordinates 3344556..3344786 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag K9U09_RS15970 Protein ID WP_000593555.1
Coordinates 3344783..3345142 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS15955 (3340593) 3340593..3343328 + 2736 WP_000149165.1 ribosome-associated ATPase/putative transporter RbbA -
K9U09_RS15960 (3343328) 3343328..3344452 + 1125 WP_001314210.1 ABC transporter permease -
K9U09_RS15965 (3344556) 3344556..3344786 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
K9U09_RS15970 (3344783) 3344783..3345142 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K9U09_RS15975 (3345262) 3345262..3345663 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
K9U09_RS15980 (3345669) 3345669..3346475 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
K9U09_RS15985 (3346472) 3346472..3347236 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
K9U09_RS15990 (3347236) 3347236..3348069 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
K9U09_RS15995 (3348066) 3348066..3349010 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T216615 WP_042014162.1 NZ_CP083497:3344556-3344786 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T216615 NZ_CP113186:200472-200756 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT216615 WP_000593555.1 NZ_CP083497:3344783-3345142 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT216615 NZ_CP113186:200234-200482 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References