Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2483242..2483837 Replicon chromosome
Accession NZ_CP083497
Organism Escherichia coli strain elppa6

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag K9U09_RS12010 Protein ID WP_000239579.1
Coordinates 2483242..2483592 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag K9U09_RS12015 Protein ID WP_001223208.1
Coordinates 2483586..2483837 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U09_RS11990 (2478518) 2478518..2479540 - 1023 WP_001339490.1 ABC transporter permease -
K9U09_RS11995 (2479554) 2479554..2481056 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
K9U09_RS12000 (2481366) 2481366..2482322 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
K9U09_RS12005 (2482632) 2482632..2483162 + 531 WP_000055072.1 inorganic diphosphatase -
K9U09_RS12010 (2483242) 2483242..2483592 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
K9U09_RS12015 (2483586) 2483586..2483837 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
K9U09_RS12020 (2484050) 2484050..2484391 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
K9U09_RS12025 (2484394) 2484394..2488173 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T216612 WP_000239579.1 NZ_CP083497:c2483592-2483242 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T216612 NZ_CP113179:205537-205821 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT216612 WP_001223208.1 NZ_CP083497:c2483837-2483586 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT216612 NZ_CP113179:205299-205547 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References