Detailed information of TA system TA216609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 1753504..1754341
• Replicon: chromosome
• Accession: NZ_CP083497
• Organism: Escherichia coli strain elppa6

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: K9U09_RS08555
• Protein ID: WP_000227784.1
• Coordinates: 1753799..1754341 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: -
• Locus tag: K9U09_RS08550
• Protein ID: WP_228596802.1
• Coordinates: 1753504..1753815 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U09_RS08525 (1748524)	1748524..1749471	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
K9U09_RS08530 (1749493)	1749493..1751484	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
K9U09_RS08535 (1751474)	1751474..1752088	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
K9U09_RS08540 (1752088)	1752088..1752417	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
K9U09_RS08545 (1752429)	1752429..1753319	+	891	WP_000971336.1	heme o synthase	-
K9U09_RS08550 (1753504)	1753504..1753815	+	312	WP_228596802.1	DUF1778 domain-containing protein	Antitoxin
K9U09_RS08555 (1753799)	1753799..1754341	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
K9U09_RS08560 (1754397)	1754397..1755332	-	936	WP_001297127.1	tetratricopeptide repeat protein	-
K9U09_RS08565 (1755740)	1755740..1757104	+	1365	WP_001000978.1	MFS transporter	-
K9U09_RS08570 (1757232)	1757232..1757723	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
K9U09_RS08575 (1757891)	1757891..1758802	+	912	WP_000705847.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T216609 WP_000227784.1 NZ_CP083497:1753799-1754341 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T216609 NZ_CP113178:c5224700-5224317 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11386.09 Da
• Isoelectric Point: 10.4825

>AT216609 WP_228596802.1 NZ_CP083497:1753504-1753815 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREVAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT216609 NZ_CP113178:c5224942-5224700 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• PDB: 7BYY