Detailed information of TA system TA216607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 703365..704003
• Replicon: chromosome
• Accession: NZ_CP083497
• Organism: Escherichia coli strain elppa6

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K9U09_RS03405
• Protein ID: WP_000813794.1
• Coordinates: 703827..704003 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K9U09_RS03400
• Protein ID: WP_001270286.1
• Coordinates: 703365..703781 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U09_RS03385 (699730)	699730..700524	-	795	WP_000555481.1	ABC transporter permease	-
K9U09_RS03390 (700514)	700514..701635	-	1122	WP_228596740.1	extracellular solute-binding protein	-
K9U09_RS03395 (701880)	701880..703286	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
K9U09_RS03400 (703365)	703365..703781	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K9U09_RS03405 (703827)	703827..704003	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K9U09_RS03410 (704225)	704225..704455	+	231	WP_000494244.1	YncJ family protein	-
K9U09_RS03415 (704547)	704547..706508	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K9U09_RS03420 (706581)	706581..707117	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
K9U09_RS03425 (707209)	707209..708384	+	1176	WP_001236319.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	708424..709689	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T216607 WP_000813794.1 NZ_CP083497:c704003-703827 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T216607 NZ_CP113178:c4755798-4755514 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT216607 WP_001270286.1 NZ_CP083497:c703781-703365 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT216607 NZ_CP113178:c4756030-4755788 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 6HPB