Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 86571..87172 Replicon plasmid unnamed5
Accession NZ_CP083496
Organism Escherichia coli strain elppa8

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag K9U11_RS25910 Protein ID WP_001216045.1
Coordinates 86792..87172 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag K9U11_RS25905 Protein ID WP_001190712.1
Coordinates 86571..86792 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U11_RS25850 (K9U11_25865) 81757..82020 + 264 WP_000224220.1 hypothetical protein -
K9U11_RS25855 (K9U11_25870) 82175..82354 + 180 Protein_89 hypothetical protein -
K9U11_RS25860 (K9U11_25875) 82442..82630 + 189 WP_032352565.1 DUF551 domain-containing protein -
K9U11_RS25865 (K9U11_25880) 82713..82946 + 234 WP_000516537.1 hypothetical protein -
K9U11_RS25870 (K9U11_25885) 83125..83418 + 294 WP_001677496.1 hypothetical protein -
K9U11_RS25875 (K9U11_25890) 83425..83799 + 375 WP_023154376.1 hypothetical protein -
K9U11_RS25880 (K9U11_25895) 83781..84713 + 933 WP_077780118.1 hypothetical protein -
K9U11_RS25885 (K9U11_25900) 84710..85072 + 363 WP_001261544.1 hypothetical protein -
K9U11_RS25890 (K9U11_25905) 85147..85284 - 138 WP_000123562.1 hypothetical protein -
K9U11_RS25895 (K9U11_25910) 85734..85985 + 252 WP_001377386.1 DNA polymerase III subunit theta -
K9U11_RS25900 (K9U11_25915) 86109..86498 + 390 WP_000506726.1 S24 family peptidase -
K9U11_RS25905 (K9U11_25920) 86571..86792 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
K9U11_RS25910 (K9U11_25925) 86792..87172 + 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
K9U11_RS25915 (K9U11_25930) 87177..87356 + 180 WP_000113019.1 hypothetical protein -
K9U11_RS25920 (K9U11_25935) 87384..88427 + 1044 WP_228596186.1 DUF968 domain-containing protein -
K9U11_RS25925 (K9U11_25940) 88516..88968 + 453 WP_001369802.1 hypothetical protein -
K9U11_RS25930 (K9U11_25945) 89055..90248 + 1194 WP_000219605.1 terminase -
K9U11_RS25935 (K9U11_25950) 90440..91732 + 1293 WP_228596188.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..110410 110410


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T216596 WP_001216045.1 NZ_CP083496:86792-87172 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T216596 NZ_CP113174:43220-43531 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT216596 WP_001190712.1 NZ_CP083496:86571-86792 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT216596 NZ_CP113174:43528-43947 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References