Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4569230..4569884 Replicon chromosome
Accession NZ_CP083492
Organism Escherichia coli strain elppa8

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag K9U11_RS22120 Protein ID WP_000244777.1
Coordinates 4569477..4569884 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K9U11_RS22115 Protein ID WP_000354046.1
Coordinates 4569230..4569496 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K9U11_RS22090 (4564399) 4564399..4565142 + 744 WP_000951964.1 SDR family oxidoreductase -
K9U11_RS22095 (4565199) 4565199..4566632 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
K9U11_RS22100 (4566677) 4566677..4566988 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
K9U11_RS22105 (4567152) 4567152..4567811 + 660 WP_000250274.1 hemolysin III family protein -
K9U11_RS22110 (4568007) 4568007..4568987 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K9U11_RS22115 (4569230) 4569230..4569496 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K9U11_RS22120 (4569477) 4569477..4569884 + 408 WP_000244777.1 protein YgfX Toxin
K9U11_RS22125 (4569924) 4569924..4570445 - 522 Protein_4313 flavodoxin FldB -
K9U11_RS22130 (4570557) 4570557..4571453 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K9U11_RS22135 (4571478) 4571478..4572188 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K9U11_RS22140 (4572194) 4572194..4573927 + 1734 WP_228595484.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T216582 WP_000244777.1 NZ_CP083492:4569477-4569884 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T216582 NZ_CP113170:26701-27006 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT216582 WP_000354046.1 NZ_CP083492:4569230-4569496 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT216582 NZ_CP113170:26481-26699 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References