Detailed information of TA system TA216581

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4437837..4438671
• Replicon: chromosome
• Accession: NZ_CP083492
• Organism: Escherichia coli strain elppa8

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: K9U11_RS21430
• Protein ID: WP_000854820.1
• Coordinates: 4437837..4438214 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: K9U11_RS21435
• Protein ID: WP_001285610.1
• Coordinates: 4438303..4438671 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K9U11_RS21395 (4433114)	4433114..4433713	+	600	WP_001255034.1	type II secretion system minor pseudopilin GspJ	-
K9U11_RS21400 (4433716)	4433716..4434693	+	978	WP_228595452.1	type II secretion system minor pseudopilin GspK	-
K9U11_RS21405 (4434690)	4434690..4435868	+	1179	WP_000094991.1	type II secretion system protein GspL	-
K9U11_RS21410 (4435870)	4435870..4436406	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
K9U11_RS21415 (4436773)	4436773..4437333	-	561	Protein_4172	DUF4942 domain-containing protein	-
K9U11_RS21420 (4437418)	4437418..4437615	-	198	WP_085949158.1	DUF957 domain-containing protein	-
K9U11_RS21425 (4437643)	4437643..4437840	-	198	Protein_4174	DUF5983 family protein	-
K9U11_RS21430 (4437837)	4437837..4438214	-	378	WP_000854820.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
K9U11_RS21435 (4438303)	4438303..4438671	-	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K9U11_RS21440 (4438751)	4438751..4438972	-	222	WP_228595453.1	DUF987 domain-containing protein	-
K9U11_RS21445 (4439059)	4439059..4439535	-	477	WP_001186193.1	RadC family protein	-
K9U11_RS21450 (4439550)	4439550..4440029	-	480	WP_228595454.1	antirestriction protein	-
K9U11_RS21455 (4440129)	4440129..4440269	+	141	WP_228595455.1	hypothetical protein	-
K9U11_RS21460 (4440295)	4440295..4441113	-	819	WP_001175155.1	DUF932 domain-containing protein	-
K9U11_RS21465 (4441203)	4441203..4441436	-	234	WP_000883181.1	DUF905 family protein	-
K9U11_RS21470 (4441442)	4441442..4442119	-	678	WP_001097565.1	hypothetical protein	-
K9U11_RS21475 (4442270)	4442270..4442950	-	681	WP_228595456.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T216581 WP_000854820.1 NZ_CP083492:c4438214-4437837 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T216581 NZ_CP113169:36656-37126 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT216581 WP_001285610.1 NZ_CP083492:c4438671-4438303 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT216581 NZ_CP113169:36216-36659 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6